Background

Deemed as a member of malignant tumors, hepatocellular carcinoma (HCC) has been characterized as a lethal disease with high morbidity and mortality. It has been widely accepted that long noncoding RNAs (lncRNAs) play a big part in the complicated biologic processes of cancer.

Aim of the study

The purpose of the study is to figure out the role and molecular regulation mechanism of ST8SIA6-AS1 in HCC.

Methods

The role of ST8SIA6-AS1 in HCC was validated by RT-qPCR, colony formation, ki-67 detection, TUNEL, JC-1 detection, wound healing and transwell-invasion assays, furthermore, the binding ability between ST8SIA6-AS1/HOXB6 and miR-5195-3p were confirmed by RNA pull down and luciferase reporter assays. Besides, the regulatory mechanism of ST8SIA6-AS1 to HOXB6/miR-5195-3p was measured by RT-qPCR and western blot assays.

Results

We measured that ST8SIA6-AS1 was highly expressed in HCC cell lines. Then knockdown of it suppressed cell proliferation, migration and migration but activated cell apoptosis in HCC. Furthermore, ST8SIA6-AS1 could bind with miR-5195-3p and negatively regulated its expression in HCC. Subsequently, it confirmed that HOXB6 was target gene of miR-5195-3p and positively modulated by ST8SIA6-AS1 in HCC. Finally, we verified that miR-5195-3p deficiency or HOXB6 upregulation countervailed the repressing effects of ST8SIA6-AS1 depletion on HCC progression.

Conclusions

To sum up, ST8SIA6-AS1 promotes HCC progression by absorbing miR-5195-3p to regulate HOXB6, which might provide some worthy suggestions to research the development process of HCC.

中文翻译：

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ST8SIA6-AS1 通过吸收 miR-5195-3p 调节 HOXB6 促进肝细胞癌。

背景

肝细胞癌（HCC）被视为恶性肿瘤的一员，已被定性为具有高发病率和死亡率的致死性疾病。人们普遍认为，长链非编码 RNA (lncRNA) 在癌症的复杂生物学过程中起着重要作用。

研究目的

本研究旨在阐明ST8SIA6-AS1在HCC中的作用及分子调控机制。

方法

ST8SIA6-AS1 在 HCC 中的作用通过 RT-qPCR、集落形成、ki-67 检测、TUNEL、JC-1 检测、伤口愈合和 transwell-invasion 检测验证，此外，ST8SIA6-AS1/HOXB6 和miR-5195-3p 通过 RNA pull down 和荧光素酶报告基因检测得到证实。此外，通过 RT-qPCR 和蛋白质印迹分析测量了 ST8SIA6-AS1 对 HOXB6/miR-5195-3p 的调节机制。

结果

我们测量到 ST8SIA6-AS1 在 HCC 细胞系中高度表达。然后它的敲低抑制了细胞增殖、迁移和迁移，但激活了 HCC 中的细胞凋亡。此外，ST8SIA6-AS1 可以与 miR-5195-3p 结合并负调节其在 HCC 中的表达。随后，证实HOXB6是miR-5195-3p的靶基因，在HCC中受ST8SIA6-AS1正调控。最后，我们证实 miR-5195-3p 缺乏或 HOXB6 上调抵消了 ST8SIA6-AS1 耗竭对 HCC 进展的抑制作用。

结论

综上所述，ST8SIA6-AS1通过吸收miR-5195-3p调控HOXB6促进HCC进展，为研究HCC的发展过程提供了一些有价值的建议。