We fabricated uniform straw-like cell architecture with central lumen using a suture thread within 1 h. The architecture consisting of cancer cells and mature adipocyte was used for cell–cell communication assay, although mature adipocyte could not form spontaneous multi-cellular spheroids. Using the system, it is possible to investigate three-dimensional cell–cell communication as an alternative to animal experiments.

Abbreviations

EB: embryoid body; 2D: two-dimension; 3D: three-dimension; PBS: phosphate buffered saline

中文翻译：

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统一的稻草样细胞架构，用于三维细胞间通信分析。

我们在1小时内使用缝合线制作了具有中央管腔的均匀稻草样细胞结构。尽管成熟的脂肪细胞不能形成自发的多细胞球体，但由癌细胞和成熟的脂肪细胞组成的体系结构用于细胞间的通讯测定。使用该系统，有可能研究三维细胞间通信，以替代动物实验。

缩略语

EB：胚状体；2D：二维；3D：三维；PBS：磷酸盐缓冲盐水