Abstract Ketoconazole was determined by the use of sequential injection analysis (SIA) in tandem with a spectrophotometric detector. This system was designed to interface to a personal computer. The proposed method is based on a coupled redox–complexation reaction, which reduces Fe(III) to Fe(II) by ketoconazole, following by the reaction of Fe(II) with 2,4,6-tripyridyl-s-triazine in an acetate buffer at pH 4.00 at the maximum absorption wavelength of 590 nm. The optimum conditions for the analysis were studied and evaluated. Beer’s law shown to be followed across the concentration range from 0.24 to 5.00 µg mL−1 with a correlation coefficient R 2 equal to 0.9962. The relative standard deviation values for the repeatability and reproducibility were less than 1.40% and 2.15% (n = 11) for 2.00 µg mL−1 of ketoconazole. The detection limit (3σ) and limit of quantification (10σ) were 0.07 and 0.24 µg mL−1, respectively. The results for the proposed method and a standard HPLC procedure provided no significant differences (p > 0.05) using Student’s t-test. Therefore, the SIA procedure has been satisfactorily applied to the determination of ketoconazole in pharmaceutical products with a sample throughput of 30 h−1. This study provides an environmentally-friendly analytical tool for quality control in the pharmaceutical industry.

中文翻译：

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绿色序贯注射分光光度法测定酮康唑

摘要 通过使用连续进样分析 (SIA) 与分光光度检测器联用来测定酮康唑。该系统设计用于连接个人计算机。所提出的方法基于耦合氧化还原络合反应，通过酮康唑将 Fe(III) 还原为 Fe(II)，然后 Fe(II) 与 2,4,6-三吡啶基-s-三嗪在pH 4.00 的醋酸盐缓冲液，最大吸收波长为 590 nm。对分析的最佳条件进行了研究和评估。啤酒定律显示在 0.24 到 5.00 µg mL-1 的浓度范围内遵循，相关系数 R 2 等于 0.9962。对于 2.00 µg mL-1 的酮康唑，重复性和再现性的相对标准偏差值分别小于 1.40% 和 2.15% (n = 11)。检测限 (3σ) 和定量限 (10σ) 分别为 0.07 和 0.24 µg mL-1。所提出的方法和标准 HPLC 程序的结果使用学生 t 检验没有提供显着差异 (p > 0.05)。因此，SIA 程序已令人满意地应用于样品通量为 30 h-1 的医药产品中酮康唑的测定。该研究为制药行业的质量控制提供了一种环保的分析工具。SIA 程序已令人满意地应用于测定药品中的酮康唑，样品通量为 30 h-1。该研究为制药行业的质量控制提供了一种环保的分析工具。SIA 程序已令人满意地应用于测定药品中的酮康唑，样品通量为 30 h-1。该研究为制药行业的质量控制提供了一种环保的分析工具。