The phorbol esters in the seeds of Jatropha curcas are a major hindrance to the full exploitation of the potential of this oil crop as a source of raw material for the production of biodiesel. Here, various quantitative proteomic strategies are used to establish the proteomes of roots, leaves, and endosperm of two genotypes of J. curcas with contrasting levels of phorbol esters in the seeds. In total 4532, 1775, and 503 proteins are identified respectively in roots, leaves, and endosperm, comprising 5068 unique proteins; of this total, 185 are differentially abundant in roots, 72 in leaves, and 20 in the endosperm. The biosynthetic pathways for flavonoids and terpenoids are well represented in roots, including the complete set of proteins for the mevalonate and non‐mevalonate/Deoxyxylulose 5‐Phosphate pathways, and proteins involved in the branches which lead to the synthesis tricyclic diterpenoids and gibberellins. Also, casbene synthase which catalyzes the first committed step in the biosynthesis of tigliane‐type diterpenes is identified in roots of both genotypes, but not in leaves and endosperm. This dataset will be a valuable resource to explore the biochemical basis of the low toxicity of Jatropha genotypes with low concentration of phorbol esters in the seeds.

中文翻译：

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与麻疯树酯水平相反的麻风树麻风树基因型的定量蛋白质组分析。

麻疯树种子中的佛波醇酯是充分利用这种油料作物作为生产生物柴油原料的潜力的主要障碍。在这里，使用各种定量蛋白质组学策略来建立麻疯树两种基因型的根，叶和胚乳的蛋白质组。种子中佛波醇酯的含量相反。在根，叶和胚乳中分别鉴定出4532、1775和503种蛋白质，其中包含5068种独特蛋白质。其中，在根中有185种差异丰富，在叶中有72种差异，在胚乳中有20差异。黄酮类化合物和萜类化合物的生物合成途径在根中得到很好的体现，包括用于甲羟戊酸和非甲羟戊酸/脱氧木酮糖5-磷酸途径的整套蛋白质，以及涉及分支的蛋白质，这些蛋白质导致合成三环二萜和赤霉素。此外，在两种基因型的根中都鉴定出可催化tigliane型二萜生物合成中第一个重要步骤的木炭合酶，但在叶和胚乳中均未鉴定出。