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Interactions and complex stabilities of grape seed procyanidins with zein hydrolysate
International Journal of Food Science & Technology ( IF 2.6 ) Pub Date : 2020-05-18 , DOI: 10.1111/ijfs.14628
Zi‐Zi Li 1 , Lei Lei 1 , Min‐Xi Huo 2 , Zi‐Rui Liu 3 , Xin‐Quan Yang 4, 5 , Yu‐Lin Wang 5 , Yang Yuan 1
Affiliation  

The objective of this paper was to investigate the complex of grape seed procyanidins (GSP) with zein hydrolysate (ZH). The interaction was determined using isothermal titration calorimetry (ITC) and fluorescence spectroscopy. The particle size, ζ‐potential, scanning electronic microscopy (SEM) and stability test of the ZH‐GSP complex were measured. The results of ITC, particle size and SEM suggested that there was a hydrogen bond‐dominated interaction between GSP and ZH, and the ZH‐GSP complex presented as a spherical shape with particle size of 234 nm, polydispersity index of 0.11 and ζ‐potential of −46.4 mV. The results of the fluorescence spectroscopy showed that the fluorescence quenching effect of GSP on ZH was GSP concentration‐dependent and the quenching process was mainly static quenching. The ZH‐GSP complex had better physical stability in aqueous solution, and their solution was more stable than GSP solution under illumination and high‐temperature treatment at 75°C.

中文翻译:

葡萄籽原花青素与玉米醇溶蛋白水解物的相互作用和复杂稳定性

本文的目的是研究葡萄籽原花青素(GSP)与玉米醇溶蛋白水解物(ZH)的复合物。使用等温滴定热法(ITC)和荧光光谱确定相互作用。测量了ZH-GSP配合物的粒径,ζ电势,扫描电子显微镜(SEM)和稳定性测试。ITC,粒径和SEM的结果表明,GSP和ZH之间存在氢键主导的相互作用,ZH-GSP配合物呈球形,粒径为234 nm,多分散指数为0.11,ζ-电势为-46.4 mV。荧光光谱结果表明,GSP对ZH的荧光猝灭作用与GSP浓度有关,猝灭过程主要为静态猝灭。
更新日期:2020-05-18
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