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Individual Nudix hydrolases affect diverse features of Pseudomonas aeruginosa.
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-05-17 , DOI: 10.1002/mbo3.1052 Joanna Drabinska 1 , Mateusz Ziecina 1 , Marta Modzelan 1 , Grazyna Jagura-Burdzy 1 , Elzbieta Kraszewska 1
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-05-17 , DOI: 10.1002/mbo3.1052 Joanna Drabinska 1 , Mateusz Ziecina 1 , Marta Modzelan 1 , Grazyna Jagura-Burdzy 1 , Elzbieta Kraszewska 1
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Nudix proteins catalyze the hydrolysis of pyrophosphate bonds in a variety of substrates and are ubiquitous in all domains of life. The genome of an important opportunistic human pathogen, Pseudomonas aeruginosa, encodes multiple Nudix proteins. To determine the role of nine Nudix hydrolases of the P. aeruginosa PAO1161 strain in its fitness, virulence or antibiotic resistance mutants devoid of individual enzymes were constructed and analyzed for growth rate, motility, biofilm formation, pyocyanin production, and susceptibility to oxidative stress and different antibiotics. The potential effect on bacterial virulence was studied using the Caenorhabditis elegans–P. aeruginosa infection model. Of the nine mutants tested, five had an altered phenotype in comparison with the wild‐type strain. The ΔPA3470, ΔPA3754, and ΔPA4400 mutants showed increased pyocyanin production, were more resistant to the β‐lactam antibiotic piperacillin, and were more sensitive to killing by H2O2. In addition, ΔPA4400 and ΔPA5176 had impaired swarming motility and were less virulent for C. elegans. The ΔPA4841 had an increased sensitivity to oxidative stress. These changes were reversed by providing the respective nudix gene in trans indicating that the observed phenotype alterations were indeed due to the lack of the particular Nudix protein.
中文翻译：
单个 Nudix 水解酶影响铜绿假单胞菌的不同特征。
Nudix 蛋白可催化多种底物中焦磷酸键的水解，并且在生命的各个领域中普遍存在。重要的机会性人类病原体铜绿假单胞菌的基因组编码多种 Nudix 蛋白。为了确定铜绿假单胞菌PAO1161 菌株的九种 Nudix 水解酶在其适应性、毒力或抗生素抗性中的作用，构建了缺乏单个酶的突变体，并分析了生长速率、运动性、生物膜形成、绿脓素产生以及对氧化应激和敏感性的影响。不同的抗生素。使用秀丽隐杆线虫-铜绿假单胞菌感染模型研究了对细菌毒力的潜在影响。在测试的九个突变体中，有五个与野生型菌株相比具有改变的表型。 ΔPA3470、ΔPA3754和ΔPA4400突变体显示出绿脓素产量增加，对β-内酰胺类抗生素哌拉西林具有更强的抗性，并且对H 2 O 2的杀伤作用更敏感。此外， ΔPA4400和ΔPA5176的集群运动能力受损，并且对秀丽隐杆线虫的毒性较低。 ΔPA4841对氧化应激的敏感性增加。通过反式提供相应的nudix基因来逆转这些变化，表明观察到的表型改变确实是由于缺乏特定的 Nudix 蛋白。
更新日期：2020-05-17
中文翻译：
单个 Nudix 水解酶影响铜绿假单胞菌的不同特征。
Nudix 蛋白可催化多种底物中焦磷酸键的水解，并且在生命的各个领域中普遍存在。重要的机会性人类病原体铜绿假单胞菌的基因组编码多种 Nudix 蛋白。为了确定铜绿假单胞菌PAO1161 菌株的九种 Nudix 水解酶在其适应性、毒力或抗生素抗性中的作用，构建了缺乏单个酶的突变体，并分析了生长速率、运动性、生物膜形成、绿脓素产生以及对氧化应激和敏感性的影响。不同的抗生素。使用秀丽隐杆线虫-铜绿假单胞菌感染模型研究了对细菌毒力的潜在影响。在测试的九个突变体中，有五个与野生型菌株相比具有改变的表型。 ΔPA3470、ΔPA3754和ΔPA4400突变体显示出绿脓素产量增加，对β-内酰胺类抗生素哌拉西林具有更强的抗性，并且对H 2 O 2的杀伤作用更敏感。此外， ΔPA4400和ΔPA5176的集群运动能力受损，并且对秀丽隐杆线虫的毒性较低。 ΔPA4841对氧化应激的敏感性增加。通过反式提供相应的nudix基因来逆转这些变化，表明观察到的表型改变确实是由于缺乏特定的 Nudix 蛋白。
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