An 8‐week feeding trial was conducted to determine dietary lysine requirement of juvenile Pseudobagrus ussuriensis (initial body weight: 0.60 g). Six isonitrogenous (crude protein, 400 g/kg) and isolipidic (crude lipid, 50 g/kg) diets were formulated to contain graded levels of dietary lysine (12.8, 19.9, 26.5, 34.0, 40.8 and 44.1 g/kg dry diets, respectively). The results indicated that weight gain, specific growth rate, productive protein value and protein efficiency ratio increased, while feed conversion ratio decreased with increasing dietary lysine level up to 34.0 g/kg dry diet and then levelled off. Fish fed diet with 12.8 g/kg lysine had the lowest lysine content (58.6 g/kg dry matter) in muscle, while fish fed diet with 34.0 g/kg lysine had the highest value (61.6 g/kg dry matter; p < .05). Broken‐line analysis on the basis of weight gain showed that the optimal dietary lysine requirement for maximum growth of juvenile Pseudobagras ussuriensis is 33.5 g/kg dry diet (82.4 g/kg dietary protein). Quadratic regression analysis of protein efficiency ratio against dietary lysine levels indicated that the optimal dietary lysine requirement of juvenile Pseudobagras ussuriensis is 36.4 g/kg dry diet (89.5 g/kg dietary protein).

中文翻译：

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乌苏假单胞菌幼虫的膳食赖氨酸需求量

进行了一个为期8周的喂养试验，以确定幼龄假单胞bag（初始体重：0.60 g）的饮食赖氨酸需求量。配制了六种同氮（粗蛋白，400 g / kg）和异脂（粗脂质，50 g / kg）日粮，以控制饮食中赖氨酸的分级水平（干饮食分别为12.8、19.9、26.5、34.0、40.8和44.1 g / kg，分别）。结果表明，随着日粮赖氨酸水平的增加，当日粮干饲料中日粮赖氨酸水平升高至34.0 g / kg时，其增重，比生长速率，生产蛋白值和蛋白效率比增加，而饲料转化率降低，然后趋于稳定。饲喂12.8 g / kg赖氨酸的鱼的肌肉中赖氨酸含量最低（58.6 g / kg干物质），而饲喂34.0 g / kg赖氨酸的鱼的肌肉中赖氨酸含量最高（61.6 g / kg干物质；p <.05）。以体重增加为基础的折线分析表明，幼鱼假单胞菌最大生长的最佳日粮赖氨酸需要量为33.5 g / kg干日粮（82.4 g / kg日粮蛋白）。蛋白质效率比对日粮赖氨酸水平的二次回归分析表明，幼小假单胞菌的最佳日粮赖氨酸需求量为36.4 g / kg干日粮（89.5 g / kg日粮蛋白）。