Four strains, coded as UPM1132, UPM1133^T, UPM1134 and UPM1135, and isolated from nodules of Pisum sativum plants grown on Ni-rich soils were characterised through a polyphasic taxonomy approach. Their 16S rRNA gene sequences were identical and showed 100% similarity with their closest phylogenetic neighbors, the species included in the ‘R. leguminosarum group’: R. laguerreae FB206^T, R. leguminosarum USDA 2370^T, R. anhuiense CCBAU 23252^T, R. sophoreae CCBAU 03386^T, R. acidisoli FH13^T and R. hidalgonense FH14^T, and 99.6% sequence similarity with R. esperanzae CNPSo 668^T. The analysis of combined housekeeping genes recA, atpD and glnII sequences showed similarities of 92-95% with the closest relatives. Whole genome average nucleotide identity (ANI) values were 97.5-99.7% ANIb similarity among the four strains, and less than 92.4% with closely related species, while digital DNA-DNA hybridization average values (dDDH) were 82-85% within our strains and 34-52% with closely related species. Major fatty acids in strain UPM1133^T were C18:1 ω7c / C18:1 ω6c in summed feature 8, C14:0 3OH/ C16:1 iso I in summed feature 2 and C18:0. Colonies were small to medium, pearl-white coloured in YMA at 28 °C and growth was observed in the ranges 8-34 °C, pH 5.5-7.5 and 0-0.7% (w/v) NaCl. The DNA G + C content was 60.8 mol %. The combined genotypic, phenotypic and chemotaxonomic data support the classification of strains UPM1132, UPM1133^T, UPM1134 and UPM1135 into a novel species of Rhizobium, for which the name Rhizobium ruizarguesonis sp. nov. is proposed. The type strain is UPM1133^T (=CECT 9542^T = LMG 30526^T).

通过多相分类方法对从生长在富镍土壤上的豌豆根瘤中分离出的四种菌株（编码为 UPM1132、UPM1133 ^T 、UPM1134 和 UPM1135）进行了表征。它们的 16S rRNA 基因序列是相同的，并且与它们最接近的系统发育邻居（包括在“R.豆科植物组'： R. laguerreae FB206 ^T 、 R. leguminosarum USDA 2370 ^T 、 R. anhuiense CCBAU 23252 ^T 、 R. sophorae CCBAU 03386 ^T 、 R. Acidisoli FH13 ^T和R. hidalgonense FH14 ^T ，与R 的序列相似性为 99.6%埃斯佩兰萨CNPSo 668 ^T 。对组合管家基因recA 、 atpD和glnII序列的分析显示，与最近的亲属有92-95%的相似性。四个菌株之间的全基因组平均核苷酸同一性 (ANI) 值为 97.5-99.7% ANIb 相似性，与密切相关的物种的相似性低于 92.4%，而我们菌株内的数字 DNA-DNA 杂交平均值 (dDDH) 为 82-85% 34-52% 与密切相关的物种有关。菌株 UPM1133 ^T中的主要脂肪酸为总结特征 8 中的 C18:1 ω7c / C18:1 ω6c、总结特征 2 中的 C14:0 3OH/ C16:1 iso I 和 C18:0。在 28 °C 的 YMA 中，菌落呈小到中等的珍珠白色，在 8-34 °C、pH 5.5-7.5 和 0-0.7% (w/v) NaCl 的范围内观察到生长。 DNA G+C含量为60.8mol%。 综合基因型、表型和化学分类学数据支持将菌株 UPM1132、UPM1133 ^T 、UPM1134 和 UPM1135 分类为根瘤菌新种，其名称为Rhizobium ruizarguesonis sp。十一月被提议。典型菌株是 UPM1133 ^T (=CECT 9542 ^T = LMG 30526 ^T )。