Biotechnological processes for the partial degradation or transformation of poly(cis-1,4-isoprene) rubber have been investigated during recent decades with promising results. The use of the enzyme 'latex clearing protein' (Lcp) to transform the polymer into more hydrophilic oligo-isoprenoids results in modifications of the rubber structure and the synthesis of new material. In order to find an alternative process to recover the degradation products, a continuous extraction method using a biphasic system is described. The enzymatic activity of Lcp1VH2 was studied in the presence of ethyl acetate and pentane as extraction solvents. Oligo(cis-1,4-isoprene) molecular species were isolated from the organic phase and analyzed by Electrospray Ionization Mass Spectrometry. The enzymatic reaction process was evaluated in terms of the biotransformation yield of poly(cis-1,4-isoprene) rubber into the corresponding degradation products. Biotransformation yields of between 42-52% were achieved depending on the enzymatic reactor design and the extraction solvent. The results also showed that the mass distribution of the oligo(cis-1,4-isoprene) depended on the organic solvent applied. A novel, simple and effective process is demonstrated for biotransformation of poly(cis-1,4-isoprene) rubber with high oligo-isoprenoid molecules recovery yields.

中文翻译：

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多相酶促反应器中聚（cis-1,4-isoprene）的生物转化，用于连续提取低聚异戊二烯分子

近几十年来，聚（顺式-1,4-异戊二烯）橡胶部分降解或转化的生物技术过程得到了研究，并取得了可喜的成果。使用“乳胶清除蛋白”(Lcp) 酶将聚合物转化为更具亲水性的低聚异戊二烯，导致橡胶结构的改变和新材料的合成。为了找到回收降解产物的替代方法，描述了使用双相系统的连续提取方法。在乙酸乙酯和戊烷作为萃取溶剂存在下研究了 Lcp1VH2 的酶活性。从有机相中分离出低聚（顺式-1,4-异戊二烯）分子种类并通过电喷雾电离质谱法进行分析。根据聚（顺式-1,4-异戊二烯）橡胶转化为相应降解产物的生物转化产率评估酶促反应过程。取决于酶反应器设计和提取溶剂，实现了 42-52% 之间的生物转化产率。结果还表明，低聚（顺式-1,4-异戊二烯）的质量分布取决于所用的有机溶剂。证明了一种新颖、简单且有效的聚（顺式-1,4-异戊二烯）橡胶生物转化方法，具有高寡聚异戊二烯分子回收率。4-异戊二烯）取决于所用的有机溶剂。证明了一种新颖、简单且有效的聚（顺式-1,4-异戊二烯）橡胶生物转化方法，具有高寡聚异戊二烯分子回收率。4-异戊二烯）取决于所用的有机溶剂。证明了一种新颖、简单且有效的聚（顺式-1,4-异戊二烯）橡胶生物转化方法，具有高寡聚异戊二烯分子回收率。