Background

Colorectal cancer (CRC) is the primary cause of cancer-related deaths worldwide. Identification of new CRC biomarkers is imperative to improve the prognosis and development of therapies against the disease. LAGE3 (L Antigen Family Member 3) functions as a tRNA modifier, although its potential role in CRC has not been fully elucidated.

Methods

RNA-seq matrix and corresponding clinical information were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, then subjected to survival, enrichment, and tumor microenvironment analyses using packages implemented in R.

Results

We found that LAGE3 was upregulated and significantly correlating with poor prognosis in multiple CRC cohorts. Additionally, multivariate Cox regression analysis revealed that LAGE3 was an independent prognostic factor in patients with CRC, whereas functional enrichment analysis indicated that it could regulate protein targeting, tRNA processing, and the PD-1/PD-L1 checkpoint pathway. Furthermore, CIBERSORT analysis indicated a negative relationship between LAGE3 and levels of infiltration for multiple immune cells, especially CD8 + T cells in CRC. Particularly, LAGE3 expression was inversely correlated with the expression of immune checkpoints as well as that of various immune cell types of signature genes.

Conclusion

Collectively, our results indicate that high LAGE3 expression correlates with adverse prognosis and poor immune infiltration in CRC patients.

中文翻译：

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LAGE3的上调与大肠癌的预后和免疫浸润相关：一种生物信息学分析。

背景

大肠癌（CRC）是全球范围内与癌症相关的死亡的主要原因。鉴定新的CRC生物标志物对于改善针对该疾病的治疗方法的预后和发展势在必行。LAGE3（L抗原家族成员3）起着tRNA修饰子的作用，尽管尚未充分阐明其在CRC中的潜在作用。

方法

从癌症基因组图谱（TCGA）和基因表达综合（GEO）数据库中下载了RNA-seq矩阵和相应的临床信息，然后使用R中实现的程序包进行了生存，富集和肿瘤微环境分析。

结果

我们发现LAGE3在多个CRC队列中被上调并与不良预后显着相关。此外，多变量Cox回归分析显示LAGE3是CRC患者的独立预后因素，而功能富集分析表明LAGE3可以调节蛋白质靶向，tRNA加工和PD-1 / PD-L1检查点途径。此外，CIBERSORT分析表明LAGE3与多种免疫细胞（尤其是CRC中的CD8 + T细胞）浸润水平之间呈负相关。特别是，LAGE3的表达与免疫检查点以及各种免疫细胞特征基因的表达呈反相关。

结论

总的来说，我们的结果表明，LAGE3高表达与CRC患者的不良预后和不良的免疫浸润相关。