Biodegradable polymeric 3D implants are of considerable interest for biomedical applications, however the degradation profile and bioactivity are important considerations for many clinical applications. In this context, bioresorbable magnesium hydroxide (MH) nanoparticles (NPs) (<50 nm) were blended with the degradable polymer poly (ε-caprolactone) (PCL) at concentrations of 5 and 20 wt%, and the composite was manufactured by 3D printing technology. Efficient load transfer was found between the nanofiller and matrix PCL, which was reflected in changes in the tensile properties of the MH-based composite. A statistically significant 44.3% increase in tensile modulus was achieved by the addition of 5 wt% MH, which was in agreement with the Halpin-Tsai theoretical model. The incorporation of MH in the PCL scaffolds accelerated the weight loss of the scaffolds and decreased the molecular weight of PCL over a prolonged soaking period (150 days) in PBS solution (pH 7.37, 37 ± 0.5 °C). The PCL/MH composite scaffolds were shown to be non-cytotoxic in vitro, and ion diffusion into the cell culture media promoted osteoblast metabolic activity, attachment, and proliferation, as compared to PCL-only scaffolds. Moreover, osteoblastic activity, as assessed by the expression of alkaline phosphatase, was significantly higher on the composite PCL/MH scaffold after 14 and 21 days. In summary, the 3D PCL/MH composite scaffolds could enhance osteoblastic activity and demonstrated a moderately accelerated degradation profile, which are characteristics that can be considered favorable for bone regeneration applications.

可生物降解的聚合物3D植入物在生物医学应用中具有相当大的意义，但是降解特性和生物活性是许多临床应用中的重要考虑因素。在这种情况下，将生物可吸收的氢氧化镁（MH）纳米颗粒（NPs）（<50 nm）与可降解的聚合物聚（ε-己内酯）（PCL）以5和20 wt％的浓度混合，然后通过3D制造复合材料印刷技术。在纳米填料和基质PCL之间发现了有效的载荷转移，这反映在MH基复合材料的拉伸性能变化中。通过添加5 wt％的MH，拉伸模量具有统计学意义的44.3％的显着增加，这与Halpin-Tsai理论模型相符。在PBS溶液（pH 7.37，37±0.5°C）中延长的浸泡时间（150天）中，MH在PCL支架中的掺入加快了支架的重量损失并降低了PCL的分子量。PCL / MH复合支架显示无细胞毒性与仅PCL支架相比，体外和离子扩散进入细胞培养基可促进成骨细胞的代谢活性，附着和增殖。此外，通过碱性磷酸酶的表达评估，成骨细胞活性在14和21天后在复合PCL / MH支架上显着更高。总而言之，3D PCL / MH复合支架可以增强成骨细胞活性并显示出适度加速的降解曲线，这些特征被认为有利于骨再生应用。