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The Immunocytochemical Identification of PIWI-Positive Cells during the Recovery of a Coelomocyte Population after Evisceration in the Holothurian Eupentacta fraudatrix (Djakonov et Baranova, 1958) (Holothuroidea: Dendrochirota)
Russian Journal of Marine Biology ( IF 0.5 ) Pub Date : 2020-05-18 , DOI: 10.1134/s106307402002011x E. G. Zavalnaya , E. V. Shamshurina , M. G. Eliseikina
中文翻译:
在Holothurian Eupentacta欺诈的内脏剔除后的白细胞数量恢复过程中,PIWI阳性细胞的免疫细胞化学鉴定(Djakonov et Baranova,1958)(Holothuroidea:Dendrochirota)
更新日期:2020-05-18
Russian Journal of Marine Biology ( IF 0.5 ) Pub Date : 2020-05-18 , DOI: 10.1134/s106307402002011x E. G. Zavalnaya , E. V. Shamshurina , M. G. Eliseikina
Abstract
The quantitative dynamics of coelomic fluid cells of Eupentacta fraudatrix (Djakonov et Baranova, 1958) were studied by flow cytometry within a day after evisceration. A differently directed dynamics pattern was noted among groups of poorly differentiated juvenile cells and differentiated cells during regeneration. The juvenile coelomocyte fraction increased, while the content of differentiated cells decreased and vice versa. The use of PIWI protein as a pluripotent stem cell marker allowed us to distinguish a PIWI-positive cell subset among juvenile coelomocytes. Their content in the coelomic fluid was highest during early regeneration at 1 and 4 h after evisceration and decreased at 24 h after evisceration.中文翻译:
在Holothurian Eupentacta欺诈的内脏剔除后的白细胞数量恢复过程中,PIWI阳性细胞的免疫细胞化学鉴定(Djakonov et Baranova,1958)(Holothuroidea:Dendrochirota)