Abstract

The quantitative dynamics of coelomic fluid cells of Eupentacta fraudatrix (Djakonov et Baranova, 1958) were studied by flow cytometry within a day after evisceration. A differently directed dynamics pattern was noted among groups of poorly differentiated juvenile cells and differentiated cells during regeneration. The juvenile coelomocyte fraction increased, while the content of differentiated cells decreased and vice versa. The use of PIWI protein as a pluripotent stem cell marker allowed us to distinguish a PIWI-positive cell subset among juvenile coelomocytes. Their content in the coelomic fluid was highest during early regeneration at 1 and 4 h after evisceration and decreased at 24 h after evisceration.

中文翻译：

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在Holothurian Eupentacta欺诈的内脏剔除后的白细胞数量恢复过程中，PIWI阳性细胞的免疫细胞化学鉴定（Djakonov et Baranova，1958）（Holothuroidea：Dendrochirota）

摘要

用内流法在流鼻血后的一天之内用流式细胞仪研究了大叶假单孢菌的腔状流体细胞的定量动力学（Djakonov et Baranova，1958）。在分化较差的幼年细胞和再生期间分化的细胞之间，观察到了不同的动力学模式。少年体腔白细胞分数增加，而分化细胞的含量减少，反之亦然。PIWI蛋白作为多能干细胞标记物的使用使我们能够区分幼年结肠细胞中PIWI阳性细胞亚群。在腔内流体中它们的含量在去内脏后1和4 h的早期再生期间最高，而在内脏后24 h下降。