Abstract

The Sea of Azov is a part of the Mediterranean basin and is the most continental sea in the world. But the phycological studies of the Sea of Azov were irregular. To assess the ecological safety of sea water, it is important not only to determine the presence of toxigenic species in the flora, but also to confirm the presence of strains capable of synthesizing toxins. The strain Nostoc linckia MZ–C21 was isolated from a planktonic sample collected near a seacoast of the Sivash (the bay of the Sea of Azov near Henichesk, Ukraine). PCR reactions demonstrated that the investigated strain has genes encoding all known groups of hepatotoxins: microcystin (the mcyE gene), nodularin (the ndaF gene), and cylindrospermopsin (the cyrB gene) synthetases. The novel strain MZ–C21 formed strongly supported phylogenetic lineages with other microcystin and nodularin producers. Thus, there is a high probability for the production of cyanotoxins by Nostoc linckia growths in the Sea of Azov near Henichesk.

中文翻译：

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亚速海的Nostoc linckia（Bornet ex Bornet et Flahault，1886年）（Nostocales：蓝细菌）：毒理学的形态学和分子研究

摘要

亚速海是地中海盆地的一部分，是世界上最大陆的海。但是亚速海的植物学研究是不规则的。为了评估海水的生态安全性，重要的是不仅要确定菌群中是否存在产毒素的物种，而且还要确认能够合成毒素的菌株的存在。Nostoc linckia MZ-C21菌株是从Sivash海岸（乌克兰Henichesk附近的Azov海海湾）附近收集的浮游样品中分离出来的。PCR反应表明，所研究的菌株具有编码所有已知肝毒素组的基因：微囊藻毒素（mcyE基因），结节菌素（ndaF基因）和cylindrospermopsin（cyrdrospermopsin）（cyrB）基因）合成酶。新型菌株MZ–C21与其他微囊藻毒素和结核菌素生产者形成了有力的系统发育谱系。因此，在Henichesk附近的亚速海中，由Nostoc linckia的生长产生氰毒素的可能性很高。