Cucumber mosaic virus (CMV) is one of the most important viral pathogen limiting banana production globally. The aim of the present study was to test the presence of CMV infection in banana plants from Ethiopia. Dot immune-binding assay and RT-PCR of symptomatic leaves could detect CMV in 92% and 100% of the samples, respectively. Direct sequencing of the PCR product produced 292 bp long nucleotide sequence showing 96.23–96.58% sequence identity at nucleotide level with isolates from Serbia (KT270566), Italy (KU976469), and others confirming CMV infection. The phylogenetic tree constructed at nucleotide level with MEGA 7.0 program revealed that the isolate belongs to subgroup IA. To the best of our knowledge, this is the first report of CMV infecting banana plants from Ethiopia.

中文翻译：

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黄瓜花叶病毒感染埃塞俄比亚'Cavendish'香蕉植物的分子检测和首次报道

黄瓜花叶病毒（CMV）是限制全球香蕉生产的最重要的病毒病原体之一。本研究的目的是测试埃塞俄比亚香蕉植物中CMV感染的存在。有斑点叶子的斑点免疫结合测定和RT-PCR分别可以检测92％和100％样品中的CMV。对PCR产物的直接测序产生了292 bp长的核苷酸序列，与来自塞尔维亚（KT270566），意大利（KU976469）和其他证实CMV感染的分离株在核苷酸水平上显示出96.23-96.58％的序列同一性。用MEGA 7.0程序在核苷酸水平上构建的系统发育树表明该分离物属于IA亚组。据我们所知，这是CMV感染埃塞俄比亚香蕉植物的第一份报告。