Endophytes colonize tissues of healthy host plants and play a crucial role in plant growth and development. However, little attention has been paid to the endophytes of tuber crops such as cassava, which is used as a staple food by approximately 800 million people worldwide. This study aimed to elucidate the diversity and composition of endophytic bacterial and fungal communities in different cassava cultivars using high-throughput sequencing. Although no significant differences in richness or diversity were observed among the different cassava cultivars, the community compositions were diverse. Two cultivars (SC124 and SC205) tolerant to root rot exhibited similar community compositions, while two other cultivars (SC10 and SC5), which are moderately and highly susceptible to root rot, respectively, harboured similar community compositions. Proteobacteria, Firmicutes, and Ascomycota dominated the endophyte assemblages, with Weissella, Serratia, Lasiodiplodia, Fusarium, and Diaporthe being the predominant genera. The differentially abundant taxonomic clades between the tolerant and susceptible cultivars were mainly rare taxa, such as Lachnoclostridium_5, Rhizobium, Lampropedia, and Stenotrophomonas. These seemed to be key genera that affected the susceptibility of cassava to root rot. Moreover, the comparison of KEGG functional profiles revealed that ‘Environmental adaptation’ category was significantly enriched in the tolerant cultivars, while ‘Infectious diseases: Parasitic’ category was significantly enriched in the susceptible cultivars. The present findings open opportunities for further studies on the roles of endophytes in the susceptibility of plants to diseases.

中文翻译：

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木薯不同品种的内生细菌和真菌菌群（Manihot esculenta Crantz）。

内生菌定植在健康宿主植物的组织中，并在植物生长和发育中起关键作用。但是，对诸如木薯之类的块茎作物的内生菌却鲜为人关注，木薯被全世界约8亿人用作主食。本研究旨在通过高通量测序阐明不同木薯品种中内生细菌和真菌群落的多样性和组成。尽管在不同的木薯品种之间未观察到丰富度或多样性的显着差异，但群落组成却是多样的。耐根腐病的两个品种（SC124和SC205）表现出相似的群落组成，而分别对根腐病具有中度和高度敏感性的另外两个品种（SC10和SC5）则具有相似的群落组成。Weissella，Serratia，Lasiodiplodia，Fusarium和Diaporthe是主要属。耐性和易感品种之间的分类进化枝差异较大，主要是稀有分类群，如Lachnoclostridium_5，根瘤菌，Lampropedia和嗜单胞菌。这些似乎是影响木薯对根腐病敏感性的关键属。此外，KEGG功能谱的比较显示，耐性品种显着丰富了“环境适应”类别，而易感品种则显着丰富了“传染病：寄生虫”类别。本发现为进一步研究内生菌在植物对疾病的敏感性中的作用提供了机会。