The enzymatic activities were indirectly investigated in the grain anthocyanin synthesis of blue barley by the HPLC data. These results were then evaluated together with absorbed light energy levels of the pigments. In addition, in two photochemical experiments, the change in aleurone pigmentation and the water activation was studied at the dormant grains, using focused sunlight and heat radiation. In the middle stage of the synthesis (at day after flowering, DAF 26), there was a significant cooling period in the weather. Differences of synthesis dynamics were found before and after the cooling period. The anthocyanin production after the cooling period (DAF 26–33) was more intense compared to the beginning of synthesis (17–22). In addition, a more intense degradation was detectable during the cooling period (22–26) than what was observed at the end of seed maturation (33–39). The most efficient light energy binders glucosides (delphinidin- and cyanidin-3-glu.) produced and degraded more dynamically than their more complex forms (malonylglucosides, rutinoside). Furthermore, among the pigments, the cyanidins are able to provide greater energy absorption. Differences in the synthesis dynamism of compounds indicate that individual enzymes and not a multienzyme complex operate in the last phase of the anthocyanin pathway, and by their operation, they can change the energy absorption level of aleurone. In irradiation of blue grains with focused sunlight (~ 400 to ~ 2500 nm, 2–3 min), the aleurone anthocyanins facilitated the vitrified water activation. During intense heat irradiation (~ 8000 nm, 1 min), the laser light scattering associated with water content decreased more intensively within the blue grains compared to the white, indicating the IR absorption surplus for the pigments. Observation suggests that the blue pigments in aleurone can contribute to energy transfer in the direction of water, so they may have role in enhancing energy dissipation.

中文翻译：

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大麦花青素途径中酶活性的表征以及这些色素对电磁辐射的反应

通过HPLC数据间接研究了蓝大麦籽粒花青素合成中的酶活性。然后将这些结果与颜料的吸收光能级一起评估。此外，在两个光化学实验中，使用聚焦的阳光和热辐射研究了休眠谷物中糊粉色素沉着和水活化的变化。在合成中期（开花后的第二天，DAF 26），天气有明显的降温期。在冷却期前后发现了合成动力学的差异。与合成开始（17-22）相比，冷却期（DAF 26-33）后花青素的产生更加强烈。此外，在冷却期 (22-26) 可以检测到比在种子成熟末期 (33-39) 观察到的更强烈的降解。最有效的光能结合剂葡萄糖苷（飞燕草素-和花青素-3-glu.）比其更复杂的形式（丙二酰葡萄糖苷、芸香苷）的产生和降解更动态。此外，在颜料中，花青素能够提供更大的能量吸收。化合物合成动力的差异表明在花青素途径的最后阶段起作用的是单个酶而不是多酶复合物，通过它们的运行，它们可以改变糊粉的能量吸收水平。在用聚焦阳光（~ 400 到~ 2500 nm，2-3 分钟）照射蓝色颗粒时，糊粉花青素促进了玻璃化水的活化。在强烈的热辐射（~ 8000 nm，1 min）期间，与白色相比，蓝色颗粒内与含水量相关的激光散射更强烈地减少，表明颜料的红外吸收过剩。观察表明糊粉中的蓝色色素可以促进能量向水方向转移，因此它们可能具有增强能量耗散的作用。