Di-(2-ethylhcxyl) phthalate (DEHP) is applied as plasticizer, which results in the pollution of environment. In this study, the effects of DEHP on soil microbial functions, structure and genetic diversity were investigated. The concentration of DEHP in the soil were 0, 0.1, 1, 10 and 50 mg/kg, and the experimental period were 28 days. DEHP reduced the quantity, abundance, species dominance and homogeneity of soil microbes during the first 14 days. In addition, microbial utilization efficiency of carbon (carbohydrates, aliphatics, amino acids, metabolites) was impacted after 28 days, though the effects gradually weakened. Based on denaturing gradient gel electrophoresis and clone library analysis, in the presence of DEHP, the dominant microbes in the DEHP-contaminated soil were Sphingomonas and Bacillus, which belonged to the Acidobacteria and Proteobacteriav, respectively. With 0.1 or 1 mg/kg of DEHP, the relative abundances of Acidobacteria were higher, and with 10 or 50 mg/kg of DEHP, the relative abundances of Proteobacteria were higher.

中文翻译：

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棕色土壤中微生物群落对邻苯二甲酸二（2-乙基己基）酯污染的响应

邻苯二甲酸二（2-乙基己基）酯（DEHP）被用作增塑剂，导致环境污染。在这项研究中，研究了DEHP对土壤微生物功能，结构和遗传多样性的影响。土壤中DEHP的浓度为0、0.1、1、10和50 mg / kg，试验期为28天。在最初的14天中，DEHP减少了土壤微生物的数量，丰富度，物种优势和同质性。此外，碳（碳水化合物，脂肪族，氨基酸，代谢产物）的微生物利用效率在28天后受到影响，尽管这种影响逐渐减弱。基于变性梯度凝胶电泳和克隆文库分析，在DEHP存在下，DEHP污染土壤中的优势微生物为鞘氨醇单胞菌和芽孢杆菌，分别属于酸性杆菌和变形杆菌。DEHP为0.1或1 mg / kg时，酸性细菌的相对丰度较高； DEHP为10或50 mg / kg时，变形杆菌的相对丰度较高。