Greenbug Schizaphis graminum is one of widespread aphid species in most wheat growing regions of Egypt that causes severe damages to crops. Studying morphological and genetic variations within insect pest populations are essential for identifying species and population distinctions and subsequently developing integrated pest management. Sixteen geographic populations of S. graminum were sampled from wheat fields in Egypt. Specimens were characterized by morphological screening for 19 quantitative morphological characters, examination of genomic DNA polymorphism with 11 inter simple sequence repeats (ISSR) primers, and measuring melting temperature (T_m) for cDNA of the mitochondrial cytochrome oxidase subunit I (COI) gene. The greatest morphological variance was observed among aphid populations from Domiata, El-Menia, and Beni-Suif. Geographic location of sampled populations had significant effect on melting temperature of the COI gene. The highest value was observed in populations from Assuit and the lowest from El-Gharbia. Populations from El-Monofia and Kafer El-Shikh were most similar based a combination of COI melting temperatures and quantitative morphological features. Tested ISSR primers showed a polymorphism rate of 49.43% among geotypes. The UBC814 primer recognized a population-specific band of 950 bp that characterized the Domiata population. Moreover, UBC811 and UBC868 primers generated bands with molecular sizes 440 and 340 bp characterizing only populations of Lower and Upper Egypt, respectively. ISSR analysis revealed that geographic populations from El-Behera, Domiata, and Kafer El-Shikh are genetically homologous with identities of 90.4–90.7%. In contrast, populations of Luxor and El-Behera are genetically analogous. Finally, a molecular branching key was constructed to discriminate among tested populations.

Greenbug Schizaphis graminum是埃及大多数小麦种植地区普遍存在的蚜虫物种之一，对农作物造成了严重破坏。研究昆虫害虫种群内的形态和遗传变异对于确定物种和种群差异并随后发展害虫综合治理至关重要。从埃及的麦田中采样了16个地理链球菌地理种群。通过对19个定量形态学特征进行形态学筛选，使用11个简单序列重复（ISSR）引物检查基因组DNA多态性以及测量解链温度（T _m）代表线粒体细胞色素氧化酶亚基I（COI）基因的cDNA。在来自Domiata，El-Menia和Beni-Suif的蚜虫种群中观察到最大的形态变异。抽样人群的地理位置对COI基因的解链温度有重要影响。在Assuit的人群中观察到最高值，在El-Gharbia的人群中观察到最低的值。基于COI熔化温度和定量形态特征的结合，来自El-Monofia和Kafer El-Shikh的种群最为相似。经测试的ISSR引物在不同地型间的多态性率为49.43％。UBC814引物识别出950 bp的种群特异性条带，该条带是Domiata种群的特征。此外，UBC811和UBC868引物产生的条带分别具有440和340 bp的分子大小，分别仅表征下埃及和上埃及人群。ISSR分析显示，来自El-Behera，Domiata和Kafer El-Shikh的地理种群在遗传上同源，同一性为90.4–90.7％。相反，卢克索（Luxor）和El-Behera的种群在遗传上相似。最后，构建了一个分子分支键来区分测试人群。