We present LIVE-PAINT, a new approach to super-resolution fluorescent imaging inside live cells. In LIVE-PAINT only a short peptide sequence is fused to the protein being studied, unlike conventional super-resolution methods, which rely on directly fusing the biomolecule of interest to a large fluorescent protein, organic fluorophore, or oligonucleotide. LIVE-PAINT works by observing the blinking of localized fluorescence as this peptide is reversibly bound by a protein that is fused to a fluorescent protein. We have demonstrated the effectiveness of LIVE-PAINT by imaging a number of different proteins inside live S. cerevisiae. Not only is LIVE-PAINT widely applicable, easily implemented, and the modifications minimally perturbing, but we also anticipate it will extend data acquisition times compared to those previously possible with methods that involve direct fusion to a fluorescent protein.

中文翻译：

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LIVE-PAINT：使用可逆肽-蛋白质相互作用在活细胞内进行超高分辨率显微镜检查

我们介绍了LIVE-PAINT，这是一种在活细胞内进行超分辨率荧光成像的新方法。在LIVE-PAINT中，仅短肽序列与正在研究的蛋白质融合，这与传统的超分辨率方法不同，传统的超分辨率方法依赖于将感兴趣的生物分子直接融合到大的荧光蛋白，有机荧光团或寡核苷酸上。LIVE-PAINT通过观察局部荧光的闪烁来工作，因为该肽被与荧光蛋白融合的蛋白可逆结合。我们通过对活啤酒酵母中的许多不同蛋白质进行成像，证明了LIVE-PAINT的有效性。LIVE-PAINT不仅广泛适用，易于实施，而且所做的修改对您的影响很小，