The objective of this study was to examine the cytotoxic effects of binary mixtures of Al2O3 and ZnO NPs using mouse fibroblast cells (L929) and human bronchial epithelial cells (BEAS-2B) as biological test systems. The synergistic, additive, or antagonistic behavior of the binary mixture was also investigated. In toxicity experiments, cellular morphology, mitochondrial function (MTT assay), apoptosis, nuclear size and shape, clonogenic assays, and damage based upon oxidative stress parameters were assessed under control and NPs exposure conditions. Although Abbott modeling results provided no clear evidence of the binary mixture of Al2O3 and ZnO NPs exhibiting synergistic toxicity, some specific assays such as apoptosis, nuclear size and shape, clonogenic assay, activities of antioxidant enzymatic enzymes catalase, superoxide dismutase, and levels of glutathione resulted in enhanced toxicity for the mixtures with 1 and 1.75 toxic units (TU) toward both cell types. Data demonstrated that co-presence of Al2O3 and ZnO NPs in the same environment might lead to more realistic environmental conditions. Our findings indicate cytotoxicity of binary mixtures of Al2O3 and ZnO NPs produced greater effects compared to toxicity of either individual compound.

中文翻译：

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Al2O3和ZnO纳米颗粒的二元混合物对成纤维细胞和支气管上皮细胞的毒性。

这项研究的目的是使用小鼠成纤维细胞（L929）和人支气管上皮细胞（BEAS-2B）作为生物学测试系统，检查Al2O3和ZnO NPs二元混合物的细胞毒性作用。还研究了二元混合物的协同，累加或拮抗行为。在毒性实验中，在对照和NPs暴露条件下评估了细胞形态，线粒体功能（MTT测定），凋亡，核大小和形状，克隆形成测定以及基于氧化应激参数的损伤。尽管Abbott建模结果没有提供明确的证据表明Al2O3和ZnO NPs的二元混合物表现出协同毒性，但是一些特定的测定方法例如凋亡，核大小和形状，克隆形成测定法，抗氧化剂酶过氧化氢酶的活性，超氧化物歧化酶，谷胱甘肽水平的升高导致对两种细胞类型具有1和1.75毒性单位（TU）的混合物的毒性增强。数据表明，在同一环境中同时存在Al2O3和ZnO NP可能导致更现实的环境条件。我们的发现表明，与任何一种化合物的毒性相比，Al2O3和ZnO NPs的二元混合物的细胞毒性产生了更大的影响。