Intrafollicular spontaneous parthenogenetic development of dromedary camel oocytes.,Molecular Reproduction and Development

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Intrafollicular spontaneous parthenogenetic development of dromedary camel oocytes.
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2020-05-16 , DOI: 10.1002/mrd.23350
Ahmed S S Abdoon ₁ , Omaima M Kandil ₁ , Shen-Ming Zeng ₂

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Dromedary camel oocytes are unique in their capability for intrafollicular and in vitro spontaneous parthenogenetic activation (SPA) and development. This study was designed for (a) observing the incidence of SPA and development of dromedary camel oocytes retrieved from ovaries; (b) assessing intrafollicular development of dromedary camel oocytes using histological examination; (c) evaluating the abilities of dromedary camel oocytes to mature, SPA, and develop in vitro; and (d) identifying the transcript abundance of Cdx2 messenger RNA (mRNA) expression in different stages of SPA and developed camel embryos. The results revealed that 2.33% of oocytes retrieved from dromedary camel ovaries were SPA and developed to blastocyst stage. Serial sections of dromedary camel ovaries also demonstrated the presence of 1.4 SPA and parthenotes per ovary, which included from two‐cell to the blastocysts with demarcated trophectoderm and inner cell mass layers. A total of 2.6% in vitro matured dromedary camel oocytes developed into morulae. The SPA and developed dromedary embryos expressed transcript abundance for Cdx2 mRNA with the highest (p < .05) at the blastocyst. The present work determines for the first time the intrafollicular oocytes from the dromedary camel display SPA, and the parthenotes can develop into blastocysts and expressing Cdx2 mRNA.

中文翻译：

单峰骆驼卵母细胞卵泡内自发孤雌发育。

单峰骆驼卵母细胞在卵泡内和体外自发孤雌生殖活化（SPA）和发育方面的能力是独特的。本研究旨在（a）观察SPA的发生率以及从卵巢中回收的单峰骆驼卵母细胞的发育；（b）使用组织学检查评估单峰骆驼卵母细胞的卵泡内发育；（c）评估单峰骆驼卵母细胞成熟，SPA和体外发育的能力；（d）确定Cdx2的转录本丰度信使RNA（mRNA）在SPA和已发育的骆驼胚胎不同阶段的表达。结果表明，从单峰骆驼卵巢中检索到的卵母细胞中有2.33％为SPA，并发育到胚泡期。单峰骆驼卵巢的连续切片还证明了每个卵巢中存在1.4 SPA和单性生殖激素，包括从两细胞到囊胚层和具有内部细胞团的囊胚。总共2.6％的体外成熟单峰骆驼卵母细胞发育为桑ula。SPA和发育的单峰胚胎的Cdx2 mRNA转录丰度最高（p <.05）。本工作首次确定了单峰骆驼展示SPA的卵泡内卵母细胞，并且单性恋者可以发育成胚泡并表达Cdx2 mRNA。

更新日期：2020-06-28

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