Sturgeon spermatozoa are unique for their sustained motility. We investigated the relative importance of bioenergetic pathways in energy supply of Siberian sturgeon Acipenser baerii spermatozoa during motile and immotile states. Spermatozoon motility and oxygen consumption rate (OCR) were analysed following exposure to inhibitors of oxidative phosphorylation [sodium azide (NaN3 )], glycolysis [2-deoxy-D-glucose (DOG)], and β-oxidation of fatty acids [sodium fluoride (NaF)] and to an uncoupler of oxidative phosphorylation [carbonyl cyanide m-chlorophenyl hydrazone (CCCP)]. No significant difference in curvilinear velocity was observed after addition of these reagents to activation medium (AM) or to non-activation medium (NAM) for incubation. Incubation of spermatozoa in NAM containing CCCP or NaN3 resulted in significantly decreased motility duration compared to controls. The OCR of sturgeon spermatozoa in AM [11.9±1.4 nmol O2 min-1 (109 spz)-1 ] was significantly higher than in NAM [8.2±1.5 nmol O2 min-1 (109 spz)-1 ]. The OCR significantly declined with addition of NaN3 to AM and NAM. No significant difference in motility parameters or OCR was observed with NaF or DOG. Results suggest active oxidative phosphorylation in both immotile and motile spermatozoa. Nevertheless, mitochondrial respiration occurring during motility is not sufficient to meet the high energy demands, and energy required for sustained motility of Siberian sturgeon spermatozoa is derived from ATP accumulated during the quiescent state. This article is protected by copyright. All rights reserved.

中文翻译：

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与濒临灭绝的西伯利亚鲟 Acipenser baerii 中持续精子运动相关的能量途径

鲟鱼精子的独特之处在于其持续的运动能力。我们研究了生物能通路在运动和不动状态下西伯利亚鲟Acipenser baerii 精子能量供应中的相对重要性。在暴露于氧化磷酸化抑制剂 [叠氮化钠 (NaN3)]、糖酵解 [2-脱氧-D-葡萄糖 (DOG)] 和脂肪酸的 β-氧化抑制剂 [氟化钠(NaF)] 和氧化磷酸化的解偶联剂 [羰基氰化物间氯苯腙 (CCCP)]。在将这些试剂添加到活化培养基 (AM) 或非活化培养基 (NAM) 进行孵育后，未观察到曲线速度的显着差异。与对照组相比，在含有 CCCP 或 NaN3 的 NAM 中培养精子导致运动持续时间显着降低。AM [11.9±1.4 nmol O2 min-1 (109 spz)-1 ]中鲟鱼精子的OCR显着高于NAM [8.2±1.5 nmol O2 min-1 (109 spz)-1]。随着向 AM 和 NAM 中添加 NaN3，OCR 显着下降。NaF 或 DOG 的运动参数或 OCR 没有显着差异。结果表明不动和活动精子中都存在活性氧化磷酸化。然而，运动期间发生的线粒体呼吸不足以满足高能量需求，西伯利亚鲟精子持续运动所需的能量来自静止状态期间积累的 ATP。本文受版权保护。版权所有。