Mechanistic insights into the reduced developmental capacity of in vitro matured oocytes and importance of cumulus cells in oocyte quality determination.,Journal of Cellular Physiology

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Mechanistic insights into the reduced developmental capacity of in vitro matured oocytes and importance of cumulus cells in oocyte quality determination.
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2020-05-16 , DOI: 10.1002/jcp.29786
Ang Li _{1,

2,

3} , Feng Wang ₃ , Li Li _{3,

4} , Li-Hua Fan _{3,

4} , Tie-Gang Meng ₃ , Qian-Nan Li ₃ , Yue Wang _{3,

4} , Wei Yue _{3,

4} , Huai-Xiu Wang ₂ , Ya-Ping Shi ₂ , Hong-Xia Li ₂ , Heide Schatten ₅ , Qing-Yuan Sun _{3,

4} , Xing-Ping Guo _{1,

2}

Affiliation

In vitro maturation of oocytes is a promising assisted reproductive technology (ART) for infertility treatment, although it is still not a routine technique for human ART due to reduced embryonic development. The aim of the present study was to clarify the possible reasons for reduced capacity of in vitro matured oocytes. Our results showed that the oocytes matured in vitro displayed increased abnormal mitochondrial distribution, reduced mitochondrial membrane potential, and increased reactive oxygen species levels when compared to in vivo matured oocytes. These results were not different in oocytes matured in vitro with or without cumulus cells. Notably, in vitro matured oocytes displayed increased mitochondrial DNA numbers probably due to functional compensation. In vitro matured oocytes showed significantly lower activation and embryonic development rates, and their ability to produce Ca²⁺ oscillations was much lower in response to parthenogenetic activation, especially in oocytes matured in vitro without cumulus cells with nearly half of them failing to produce calcium waves upon strontium chloride stimulation. These data are important for understanding the reasons for reduced developmental potential of in vitro matured oocytes and the importance of cumulus cells for oocyte quality.

中文翻译：

对体外成熟卵母细胞发育能力下降以及卵丘细胞在卵母细胞质量测定中的重要性的机械见解。

卵母细胞的体外成熟是一种有前途的不育治疗辅助生殖技术（ART），尽管由于减少了胚胎发育，它仍然不是人类ART的常规技术。本研究的目的是阐明体外成熟卵母细胞能力降低的可能原因。我们的结果表明，与体内成熟的卵母细胞相比，体外成熟的卵母细胞显示出异常的线粒体分布增加，线粒体膜电位降低和活性氧水平升高。这些结果在有卵细胞或无卵细胞的体外成熟卵母细胞中没有差异。值得注意的是，体外成熟的卵母细胞显示线粒体DNA数量增加可能是由于功能补偿所致。²⁺振荡对孤雌生殖活化的响应要低得多，尤其是在没有卵丘细胞的体外成熟卵母细胞中，近一半的卵母细胞在氯化锶刺激下不能产生钙波。这些数据对于理解体外成熟卵母细胞发育潜力降低的原因以及卵丘细胞对卵母细胞质量的重要性至关重要。

更新日期：2020-05-16

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