The potential toxicity to human and aquatic receptors of petroleum fuel biodegradation metabolites (oxygen‐containing organic compounds [OCOCs]) in groundwater has been investigated as part of a multi‐year research program. Whole mixtures collected from locations upgradient and downgradient of multiple fuel release sites were tested using: 1) in vitro screening assays for human genotoxicity (the gamma‐H2AX assay) and estrogenic effects (estrogen receptor transcriptional activation assay), and 2) chronic aquatic toxicity tests in 3 species (Ceriodaphnia dubia , Raphidocelis subcapitata , and Pimephales promelas ). In vitro screening assay results demonstrated that the mixtures did not cause genotoxic or estrogenic effects. No OCOC‐related aquatic toxicity was observed and when aquatic toxicity did occur, upgradient samples typically had the same response as samples downgradient of the release, indicating that background water quality was impacting the results. This information provides additional support for previous work that focused on the individual compounds and, taken together, indicates that OCOCs from petroleum degradation at fuel release sites are unlikely to cause toxicity to human or freshwater receptors at the concentrations present. Environ Toxicol Chem 2020;39:1634–1645. © 2020 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

中文翻译：

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燃料释放场所地下水中石油生物降解代谢物混合物对人类和水生的潜在毒性。

作为一项多年研究计划的一部分，已经研究了地下水中石油燃料生物降解代谢物（含氧有机化合物[OCOCs]）对人类和水生受体的潜在毒性。从多个燃料释放部位的上升和下降位置收集的全部混合物使用以下方法进行测试：1）体外筛选测定人类遗传毒性（γ-H2AX测定）和雌激素作用（雌激素受体转录激活测定），以及2）慢性水生毒性在3种物种中进行了检测（百色蜡嘴鸦藻（Ceriodaphnia dubia），头兜螺（Raphidocelis subcapitata）和吡美pha（Pimephales promelas））。体外筛选测定结果表明，该混合物没有引起遗传毒性或雌激素作用。没有观察到与OCOC有关的水生毒性，当确实发生水生毒性时，上级样品通常具有与释放的下级样品相同的响应，表明背景水质正在影响结果。该信息为以前针对单个化合物的研究提供了额外的支持，这些研究加在一起表明，在存在的浓度下，来自石油在燃料释放部位降解的OCOC不太可能对人或淡水受体产生毒性。2020年《环境毒理学》； 39：1634–1645。©2020作者。环境毒理学与化学 由Wiley Periodicals LLC代表SETAC发行。