The two most common Trichinella species present in European countries are Trichinella spiralis and Trichinella britovi. The consumption of raw or undercooked meat with invasive larvae results in trichinellosis. Currently, the most commonly used sources for detecting specific anti-Trichinella antibodies is ELISA with the muscle larvae (ML) excretory-secretory (E-S) proteins. However, these serological methods cannot be efficiently applied at early stage of infection. The aim of the current study was to identify the common and species-specific E-S proteins of T. spiralis and T. britovi adult worms which could have potential for accurate diagnosis of Trichinella infection at an early stage of invasion. Different sets of immunoreactive proteins were identified in T. spiralis and T. britovi proteomes by a combination of two-dimensional electrophoresis (2-DE), immunoblot and LC-MS/MS analysis. Polyubiquitin-B, a possible enoyl-CoA hydratase/isomerase YngF or polyubiquitin-like protein was found to be common for both species; gene ontology analysis confirmed its involvement in proteolysis, oxidation-reduction and translation processes, as well as in molecular transport. These molecules, being secreted or excreted at an early stage of parasite development, may play a critical role in the processes occurring during the initial steps of the host invasion and hence be suitable for diagnostic test development.

存在于欧洲国家的两种最常见的旋毛虫物种是旋毛虫和布氏旋毛虫。食用带有侵入性幼虫的生肉或未煮熟的肉会导致旋毛虫病。目前，检测特定抗旋毛虫抗体的最常用来源是使用肌肉幼虫 (ML) 排泄-分泌 (ES) 蛋白的 ELISA。然而，这些血清学方法不能有效地应用于感染的早期阶段。当前研究的目的是确定共同的和种属特异性ES的蛋白质旋毛虫和T. britovi这有可能给准确诊断潜在的成虫旋毛虫在入侵的早期阶段感染。在鉴定不同组的免疫反应性蛋白质的旋毛虫和T. britovi通过二维电泳（2-DE），免疫印迹和LC-MS / MS分析的组合蛋白质组。聚泛素-B，一种可能的烯酰辅酶A水合酶/异构酶YngF或聚泛素样蛋白被发现在这两种物种中都很常见；基因本体分析证实其参与蛋白水解、氧化还原和翻译过程，以及分子转运。这些分子在寄生虫发育的早期阶段被分泌或排泄，可能在宿主入侵的初始阶段发生的过程中发挥关键作用，因此适用于诊断测试的开发。