Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.

无症状的户外犬可能是犬巴贝斯病的携带者，但尚无描述急性期反应（APR）发展的数据。我们假设这些狗具有适度的APR，可以通过血液学和生化变化检测到。两组巴贝-exposed犬，九代表B.犬PCR阳性二十B.犬PCR阴性，狗的血清阳性。对照组由十个巴贝虫组成天真的狗。用标准方法分析血清淀粉样蛋白A（SAA），对氧磷酶-1（PON-1），全血细胞计数和生化参数。使用琼脂糖凝胶电泳（GE）分离蛋白质和脂蛋白组分，并在梯度GE上评估脂蛋白的主要直径。使用非参数测试和接收器工作特性曲线评估结果。SAA（中值39.0μg/ mL，范围2.2–48.8μg/ mL），总蛋白（中值74.7 g / L，范围57.1–98.3 g / L）和α-脂蛋白的主要直径（中值13.31 nm，范围12.09–）犬B.canis PCR阳性犬中的14.17 nm）相对于对照组中的犬或PCR阴性但具有血清反应活性（p 两组均<0.001）。这是接近参考范围的上限轻度至中度贫血（4/29），血小板减少症（7/29），和白细胞计数均遇到了巴贝-exposed组。与对照组相比，暴露于巴贝虫的狗表现出的PON-1活性和蛋白GE模式降低，与低度慢性炎症一致（ 两组均p <0.001）。犬血液中犬双歧杆菌DNA含量可检测的狗含有较高水平的SAA和总蛋白以及α-脂蛋白，相对于那些患有巴比虫血清学阳性但犬血液中双歧杆菌DNA含量无法检测的狗，其脂蛋白的直径增加。