Azithromycin and diminazene aceturate combination therapy in experimental multidrug-resistant Trypanosoma brucei brucei infection in albino rats was evaluated. A total of forty-five female albino rats were used. These rats were randomly assigned to nine groups of five rats each. Group 1 was the uninfected-untreated group while groups 2 - 6 were infected with 1 × 10⁶ trypanosomes suspended in 0.3 ml of normal saline intraperitoneally. Following infection and parasitaemia, group 2 was untreated while group 3 was treated once with 7 mg/kg diminazene aceturate. Groups 4 - 6 were treated with 10, 20 and 30 mg/kg azithromycin respectively for 7 days. Groups 7 - 9 were treated with combination of 7 mg/kg diminazene aceturate (DA) once and 10, 20 and 30 mg/kg azithromycin (AZT) respectively for 7 days. Level of parasitaemia, haematological indices (packed cell volume, total erythrocyte count, total leukocyte count, haemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration), survivability, body weight and rectal temperature were used to assess the effectiveness of the combination therapy. A significant reduction in parasitaemia levels was observed in the DA-treated group and AZT-treated group 6 while clearance of parasitaemia was observed in the DA-AZT treated groups 7 – 9 for periods between 1 and 5 days post treatment. The haematological indices and survivability of the DA-AZT treated groups were better than the DA-treated group despite the relapse recorded in those groups. One rat each in the DA-AZT combination groups survived till the end of the experiment. In conclusion, the DA-AZT combination treatment can be used as a possible adjunct to DA in the treatment of multidrug-resistant T. brucei brucei. The combination also enhanced survivability and decreased the effect of the disease in rats.

评价了阿奇霉素和醋酸地米那嗪联合治疗白化病大鼠多药耐药布鲁氏布鲁氏菌感染的实验方法。总共使用了四十五只雌性白化病大鼠。将这些大鼠随机分为九组，每组五只。第1组是未感染的未治疗组，而第2-6组则感染了1×10 ⁶锥虫腹膜内悬浮于0.3 ml生理盐水中。感染和寄生虫血症后，不对第2组进行治疗，而对第3组用7 mg / kg醋酸二咪唑治疗一次。4-6组分别用10、20和30 mg / kg阿奇霉素治疗7天。第7-9组分别用7 mg / kg醋酸二咪唑（DA）和10、20和30 mg / kg阿奇霉素（AZT）组合治疗7天。使用寄生虫血症水平，血液学指标（堆积细胞体积，总红细胞计数，总白细胞计数，血红蛋白浓度，平均红细胞体积，平均红细胞血红蛋白，平均红细胞血红蛋白浓度），存活率，体重和直肠温度来评估联合疗法。在DA治疗组和AZT治疗组6中，寄生虫血症水平显着降低，而DA-AZT治疗组7-9在治疗后1至5天观察到寄生虫血症的清除率。尽管DA-AZT治疗组有复发，但血液学指标和存活率仍优于DA治疗组。DA-AZT组合组中的每只大鼠都存活到实验结束。总之，DA-AZT联合治疗可以作为多药耐药的DA的辅助治疗 尽管DA-AZT治疗组有复发，但血液学指标和存活率仍优于DA治疗组。DA-AZT组合组中的每只大鼠都存活到实验结束。总之，DA-AZT联合治疗可以作为多药耐药的DA的辅助治疗 尽管DA-AZT治疗组有复发，但血液学指标和存活率仍优于DA治疗组。DA-AZT组合组中的每只大鼠都存活到实验结束。总之，DA-AZT联合治疗可作为多药耐药的DA的辅助治疗T. brucei brucei。该组合还增强了大鼠的生存能力并降低了该疾病的影响。