Experimental rabbits provide evidence for translational research regarding the pathogenies or treatment of human diseases. We developed a novel method for regenerating the middle ear mucosa using autologous cultured nasal mucosal epithelial cell sheets, and evaluated the wound healing process in the middle ear mucosa of experimental rabbits. Nonetheless, vigilant microbiological monitoring of experimental animals is essential to effectively prevent a decline in their health conditions, which may affect the research results. We experimented with contamination of Pasteurella multocida in non-specific-pathogen-free (SPF) rabbits (without microbiological monitoring). Most non-SPF rabbits had otitis media, whereas SPF rabbits did not, which affected their results during the mucosal regeneration study. The contamination was resolved by changing the experimental design from using non-SPF rabbits to that using SPF rabbits. It is crucial to use the SPF animals for any surgical intervention studies.

实验性兔子为有关人类疾病的病因或治疗的转化研究提供了证据。我们开发了一种使用自体培养的鼻黏膜上皮细胞片再生中耳黏膜的新方法，并评估了实验兔中耳黏膜的伤口愈合过程。尽管如此，对实验动物进行警惕的微生物监测对于有效防止其健康状况的下降至关重要，因为健康状况的下降可能会影响研究结果。我们实验了多杀性巴氏杆菌的污染在非特异性无病原体（SPF）兔中进行（无微生物监测）。大多数非SPF兔患有中耳炎，而SPF兔则没有，这会影响其在粘膜再生研究中的结果。通过将实验设计从使用非SPF兔改为使用SPF兔，可以解决污染问题。使用SPF动物进行任何外科手术干预研究至关重要。