Circular RNAs (circRNAs) act as essential regulators in the tumorigenesis of renal cell carcinoma. Our study aims to investigate the underlying function and the molecular mechanisms of circ_0001368 in renal cell carcinoma. The qRT-PCR results indicate that the circ_0001368 expression level was downregulated in the carcinoma cells and tissues of the renal cell. circ_0001368 weakened cell proliferation and invasion in the ACHN and 786-O cells. The luciferase reporter assay showed that circ_0001368 functioned as an endogenous sponge for miR-492. The transwell and CCK8 assays showed that circ_0001368 suppressed cell proliferation and invasion in the ACHN and 786-O cells. Large tumor suppressor kinase 2 (LATS2) was confirmed by Targetscan as a target gene of miR-492. The overexpression of LATS2 repressed the growth and invasion of ACHN and 786-O cells. circ_0001368 upregulated the LATS2 expression and suppressed ACHN and 786-O cell growth and invasion by sponging miR-492. circ_0001368 suppressed the proliferation ability of 786-O in vivo. In conclusion, circ_0001368 was identified in this study as a novel anti-tumor RNA in renal cell carcinoma and can function as a potential therapeutic target for renal cell carcinoma treatment.

中文翻译：

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环状RNA circ_0001368通过使miR-492海绵化并靶向LATS2抑制肾细胞癌的生长和侵袭。

环状RNA（circRNA）在肾细胞癌的肿瘤发生中起着重要的调节作用。我们的研究旨在探讨circ_0001368在肾细胞癌中的潜在功能及其分子机制。qRT-PCR结果表明在癌细胞和肾细胞组织中circ_0001368表达水平下调。circ_0001368减弱了ACHN和786-O细胞中的细胞增殖和侵袭。荧光素酶报告基因测定表明，circ_0001368充当miR-492的内源海绵。Transwell和CCK8分析表明circ_0001368抑制了ACHN和786-O细胞中的细胞增殖和侵袭。Targetscan确认了大肿瘤抑制激酶2（LATS2）是miR-492的靶基因。LATS2的过表达抑制了ACHN和786-O细胞的生长和侵袭。circ_0001368上调了miR-492的含量，从而上调了LATS2的表达并抑制了ACHN和786-O细胞的生长和侵袭。circ_0001368抑制了786-O在体内的增殖能力。总之，在本研究中，circ_0001368被鉴定为肾细胞癌中的一种新型抗肿瘤RNA，可以作为肾细胞癌治疗的潜在治疗靶标。