当前位置：
X-MOL 学术
›
Acta Trop.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Investigation of antileshmanial activities of Cuminum cyminum based green silver nanoparticles on L. tropica promastigotes and amastigotes in vitro.
Acta Tropica ( IF 2.1 ) Pub Date : 2020-05-16 , DOI: 10.1016/j.actatropica.2020.105498 Melahat Bagirova 1 , Sahar Dinparvar 1 , Adil M Allahverdiyev 1 , Kubra Unal 1 , Emrah Sefik Abamor 1 , Metanet Novruzova 2
Acta Tropica ( IF 2.1 ) Pub Date : 2020-05-16 , DOI: 10.1016/j.actatropica.2020.105498 Melahat Bagirova 1 , Sahar Dinparvar 1 , Adil M Allahverdiyev 1 , Kubra Unal 1 , Emrah Sefik Abamor 1 , Metanet Novruzova 2
Affiliation
|
Leishmaniasis is one of the most important parasitic diseases, which is caused by Leishmania species. Nowadays; although pentavalent antimonials are used as the basic treatment option for Cutaneous Leishmaniasis, high cost, toxicity and resistance of the parasites to the medication over time are some important limitations causing several problems in the treatment. In recent years, the progress in the field of green nanotechnology provides the development of green nanoparticle-based treatment methods for Cutaneous Leishmaniasis. The importance of green nanoparticles has gradually increased due to their special reductive, stabilizing, antioxidant and non-toxic properties. Although there are many studies based on green nanoparticles against Leishmania parasites, we have not found any research about antileishmanial activities of biosynthesized silver nanoparticles (Bio-AgNPs) using Cuminum cyminum L (Cumin) seed extract. Therefore for the first time in this study in vitro antileishmanial effects of Bio-AgNPs prepared from Cumin seed extract were examined on L. tropica promastigote and amastigote forms and their efficacies were compared with chemically synthetized AgNPs. During the experiments, antileishmanial effects of synthetized nanoparticles were determined on both promastigote and amastigote forms of Leishmania parasites by detecting different parameters such as proliferation, infection index and produced nitric oxide (NO) amounts from macrophages. According to the results, it was shown that Bio-AgNPs and AgNPs excessively inhibited L. tropica promastigotes and amastigotes by significantly decreasing proliferation rates of promastigotes and metabolic activities of amastigotes, as well. Moreover, infection index rates of macrophages revealed remarkable anti-amastigote performances of Bio-AgNPs. Besides, Bio-AgNPs stimulated macrophages to release NO to kill Leishmania parasites. Consequently, for the first time, Bio-AgNPs were found to be more effective on both forms of Leishmania parasites than AgNPs. Obtained results indicated high antileishmanial potential of green nanoparticles on L. tropica parasites, causative agents of Cutaneous Leishmaniasis. Thus, obtained results demonstrated that green nanoparticles can lead to the development of new, safer, stable and more effective antileishmanial formulations against Cutaneous Leishmaniasis.
中文翻译：
基于小茴香的绿色银纳米颗粒对热带乳杆菌前鞭毛体和无鞭毛体的体外抗利什曼活性的研究。
利什曼病是最重要的寄生虫病之一，由利什曼原虫引起。如今;尽管五价锑被用作皮肤利什曼病的基本治疗选择，但成本高、毒性和寄生虫随着时间的推移对药物的耐药性是一些重要的限制，导致治疗中出现一些问题。近年来，绿色纳米技术领域的进展为基于绿色纳米粒子的皮肤利什曼病治疗方法的发展提供了基础。绿色纳米粒子由于其特殊的还原性、稳定性、抗氧化性和无毒特性，其重要性逐渐增加。尽管有许多基于绿色纳米粒子对抗利什曼原虫寄生虫的研究，但我们尚未发现任何关于使用孜然种子提取物生物合成的银纳米粒子（Bio-AgNP）的抗利什曼原虫活性的研究。因此，本研究首次在体外检测了由孜然籽提取物制备的生物银纳米颗粒对热带乳杆菌前鞭毛体和无鞭毛体形式的抗利什曼病作用，并将其功效与化学合成的银纳米颗粒进行了比较。在实验过程中，通过检测增殖、感染指数和巨噬细胞产生的一氧化氮（NO）量等不同参数，确定了合成纳米颗粒对利什曼原虫寄生虫的前鞭毛体和无鞭毛体形式的抗利什曼效应。结果表明，Bio-AgNPs和AgNPs通过显着降低前鞭毛体的增殖率和无鞭毛体的代谢活性来过度抑制热带乳杆菌前鞭毛体和无鞭毛体。 此外，巨噬细胞的感染指数率显示出Bio-AgNPs显着的抗无鞭毛体性能。此外，Bio-AgNPs 刺激巨噬细胞释放 NO 来杀死利什曼原虫。因此，首次发现 Bio-AgNP 对两种形式的利什曼原虫寄生虫都比 AgNP 更有效。获得的结果表明绿色纳米颗粒对热带利什曼原虫（皮肤利什曼病的病原体）具有很高的抗利什曼病潜力。因此，获得的结果表明，绿色纳米颗粒可以导致开发新的、更安全、稳定和更有效的抗皮肤利什曼病制剂。
更新日期：2020-05-16
中文翻译：
基于小茴香的绿色银纳米颗粒对热带乳杆菌前鞭毛体和无鞭毛体的体外抗利什曼活性的研究。
利什曼病是最重要的寄生虫病之一，由利什曼原虫引起。如今;尽管五价锑被用作皮肤利什曼病的基本治疗选择，但成本高、毒性和寄生虫随着时间的推移对药物的耐药性是一些重要的限制，导致治疗中出现一些问题。近年来，绿色纳米技术领域的进展为基于绿色纳米粒子的皮肤利什曼病治疗方法的发展提供了基础。绿色纳米粒子由于其特殊的还原性、稳定性、抗氧化性和无毒特性，其重要性逐渐增加。尽管有许多基于绿色纳米粒子对抗利什曼原虫寄生虫的研究，但我们尚未发现任何关于使用孜然种子提取物生物合成的银纳米粒子（Bio-AgNP）的抗利什曼原虫活性的研究。因此，本研究首次在体外检测了由孜然籽提取物制备的生物银纳米颗粒对热带乳杆菌前鞭毛体和无鞭毛体形式的抗利什曼病作用，并将其功效与化学合成的银纳米颗粒进行了比较。在实验过程中，通过检测增殖、感染指数和巨噬细胞产生的一氧化氮（NO）量等不同参数，确定了合成纳米颗粒对利什曼原虫寄生虫的前鞭毛体和无鞭毛体形式的抗利什曼效应。结果表明，Bio-AgNPs和AgNPs通过显着降低前鞭毛体的增殖率和无鞭毛体的代谢活性来过度抑制热带乳杆菌前鞭毛体和无鞭毛体。 此外，巨噬细胞的感染指数率显示出Bio-AgNPs显着的抗无鞭毛体性能。此外，Bio-AgNPs 刺激巨噬细胞释放 NO 来杀死利什曼原虫。因此，首次发现 Bio-AgNP 对两种形式的利什曼原虫寄生虫都比 AgNP 更有效。获得的结果表明绿色纳米颗粒对热带利什曼原虫（皮肤利什曼病的病原体）具有很高的抗利什曼病潜力。因此，获得的结果表明，绿色纳米颗粒可以导致开发新的、更安全、稳定和更有效的抗皮肤利什曼病制剂。
京公网安备 11010802027423号