Bladder cancer is one of the commonest malignant tumors of urinary system with high recurrence. However, currently developed bladder cancer urine diagnosis methods are hindered by the low detection sensitivity and accuracy. Herein, a molybdenum disulfide (MoS₂) nanosheets-based field effect transistor (FET) sensor array was constructed for simultaneous detection of multiple bladder cancer biomarkers in human urine. With the excellent electronic property of MoS₂ and the high specific identification capability of recognition molecules, the proposed biosensor array could simultaneously detect nuclear matrix protein 22 (NMP22) and cytokeratin 8 (CK8) with a wide linear range of 10⁻⁶−10⁻¹ pg mL⁻¹ and an ultra-low detection limit of 0.027 and 0.019 aM, respectively. Furthermore, this highly sensitive and specific MoS₂ FET sensor array could be used to identify bladder cancer biomarkers from human urine samples. This designed high-performance biosensor array shows great potential in the future diagnosis of bladder cancer.

膀胱癌是泌尿系统最常见的高复发性恶性肿瘤之一。然而，目前开发的膀胱癌尿液诊断方法由于检测灵敏度和准确性低而受到阻碍。本文中，构建了基于二硫化钼（MoS ₂）纳米片的场效应晶体管（FET）传感器阵列，用于同时检测人尿液中的多种膀胱癌生物标记物。凭借MoS ₂的出色电子性能和识别分子的高特异性识别能力，该生物传感器阵列可以同时检测线性范围为10 ^-6 -10-的核基质蛋白22（NMP22）和细胞角蛋白8（CK8）^{。 1} pg毫升^-1检测极限分别为0.027和0.019 aM。此外，这种高度灵敏且特异的MoS ₂ FET传感器阵列可用于从人尿液样本中识别膀胱癌生物标志物。这种设计的高性能生物传感器阵列在膀胱癌的未来诊断中显示出巨大的潜力。