The present study aims to investigate the association between SOX4, Wnt signaling, and miRNAs under Wnt3 induction via bioinformatics analysis and functional essays. To briefly explore the expression of SOX4 protein in various types of cancer, we used ONCOMINE, a highly reputable cancer database, for comparison of its expression in prostate carcinoma relative to normal prostate gland. Concomitantly, we used CCLE to plot the copy number of SOX4 against its mRNA expression status in various cancerous cell lines to confirm the carcinogenesis role of SOX4. Afterward, whole profiling expression of microRNA in SOX4-stably expressed LNCaP cell line under the effect of Wnt3A were demonstrated. After identifying microRNA targets, STRING database and MIROB were used to explore the functional connection between proteins and microRNA with proteins. The results from our study shows that over-expressed of SOX4 was confirmed in both carcinogenesis tissue and cancer cell lines in Oncomine and CCLE database. In addition, five miRNAs, miR-16, miR-19a, miR320, miR-195, and miR-126, were differentially expressed in LNCaP cell line induced by Wnt3a. Pathway analysis of these targets proposed interaction networks of SOX4, Wnt3a with miR-126 and miR-195. Altogether, the miRNAs involved in Wnt and SOX4-mediated prostate cancer such as miR-126 and miR-195 could be potential biomarkers in prostate cancer.

中文翻译：

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SOX4 的过表达诱导 LNCaP 前列腺癌细胞系中 miR-126 和 miR-195 的上调。

本研究旨在通过生物信息学分析和功能论文研究 Wnt3 诱导下 SOX4、Wnt 信号传导和 miRNA 之间的关联。为了简要探讨 SOX4 蛋白在各种类型癌症中的表达，我们使用了信誉良好的癌症数据库 ONCOMINE 来比较其在前列腺癌中相对于正常前列腺的表达。同时，我们使用 CCLE 将 SOX4 的拷贝数与其在各种癌细胞系中的 mRNA 表达状态作图，以确认 SOX4 的致癌作用。然后，证明了在 Wnt3A 的作用下，microRNA 在 SOX4 稳定表达的 LNCaP 细胞系中的全谱表达。确定microRNA靶点后，利用STRING数据库和MIROB探索蛋白质与microRNA与蛋白质之间的功能联系。我们的研究结果表明，在 Oncomine 和 CCLE 数据库中的致癌组织和癌细胞系中都证实了 SOX4 的过度表达。此外，五种 miRNA，miR-16、miR-19a、miR320、miR-195 和 miR-126 在 Wnt3a 诱导的 LNCaP 细胞系中差异表达。这些靶标的通路分析提出了 SOX4、Wnt3a 与 miR-126 和 miR-195 的相互作用网络。总之，参与 Wnt 和 SOX4 介导的前列腺癌的 miRNA，如 miR-126 和 miR-195，可能是前列腺癌的潜在生物标志物。这些靶标的通路分析提出了 SOX4、Wnt3a 与 miR-126 和 miR-195 的相互作用网络。总之，参与 Wnt 和 SOX4 介导的前列腺癌的 miRNA，如 miR-126 和 miR-195，可能是前列腺癌的潜在生物标志物。这些靶标的通路分析提出了 SOX4、Wnt3a 与 miR-126 和 miR-195 的相互作用网络。总之，参与 Wnt 和 SOX4 介导的前列腺癌的 miRNA，如 miR-126 和 miR-195，可能是前列腺癌的潜在生物标志物。