With its rare and naturally blue corolla, Plumbago auriculata Lam. has received attention and been introduced and planted worldwide. However, it is sensitive to low temperatures. Continuous low temperatures cause the stagnation of plant growth and the disappearance of ornamental traits. Currently, there is no report on the response of P. auriculata to low temperature. In this study, the chlorophyll fluorescence parameters of P. auriculata leaves were analysed after 10 days of low-temperature stress, and 943 differentially expressed genes were identified via high-throughput RNA sequencing. P. auriculata copes with low-temperature stress by changing the pathways of sugar metabolism, glutathione metabolism and lipid metabolism; however, the decrease in the assimilation level caused by damage to the photoreaction system is an important reason for plant growth stagnation. In addition, we identified 47 differentially expressed transcription factors, including the upregulated MYBS3 transcription factor, which is involved in the MYBS3-dependent pathway that is distinct from the DREB/CBF pathway. Our data reveal the cause of P. auriculata stagnation at low temperatures and provide valuable information for further studies on cold-response mechanisms and the genetic improvement of cold tolerance in P. auriculata.

以其稀有的天然蓝色花冠Plumbago auriculata Lam。已受到关注，并已在全球范围内引入和种植。但是，它对低温敏感。持续的低温导致植物生长停滞，观赏性状消失。目前，尚无关于光合假单胞菌对低温的反应的报道。在这项研究中，在低温胁迫10天后分析了光合紫苏叶的叶绿素荧光参数，并通过高通量RNA测序鉴定了943个差异表达基因。uri通过改变糖代谢，谷胱甘肽代谢和脂质代谢的途径来应对低温胁迫；然而，由于光反应体系的破坏而导致的同化水平降低是植物生长停滞的重要原因。此外，我们鉴定了47种差异表达的转录因子，包括上调的MYBS3转录因子，该因子参与了与DREB / CBF途径不同的MYBS3依赖性途径。我们的数据显示的原因P.蕨停滞在低温和提供冷反应机制和耐冷性的遗传改良进一步研究的有价值的信息P.蕨。