Role for Cell-Surface Collagen of Streptococcus pyogenes in Infections.,ACS Infectious Diseases

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Role for Cell-Surface Collagen of Streptococcus pyogenes in Infections.
ACS Infectious Diseases ( IF 4.0 ) Pub Date : 2020-05-15 , DOI: 10.1021/acsinfecdis.0c00073
Aubrey J Ellison , Felix Dempwolff ₁ , Daniel B Kearns ₁ , Ronald T Raines ₂

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Group A Streptococcus (GAS) displays cell-surface proteins that resemble human collagen. We find that a fluorophore-labeled collagen mimetic peptide (CMP) labels GAS cells but not Escherichia coli or Bacillus subtilis cells, which lack such proteins. The CMP likely engages in a heterotrimeric helix with endogenous collagen, as the nonnatural d enantiomer of the CMP does not label GAS cells. To identify a molecular target, we used reverse genetics to “knock-in” the GAS genes that encode two proteins with collagen-like domains, Scl1 and Scl2, into B. subtilis. The fluorescent CMP labels the cells of these B. subtilis strains. Moreover, these strains bind tightly to a surface of mammalian collagen. These data are consistent with streptococcal collagen forming triple helices with damaged collagen in a wound bed and thus have implications for microbial virulence.

中文翻译：

化脓性链球菌的细胞表面胶原蛋白在感染中的作用。

A组链球菌（GAS）显示类似于人胶原蛋白的细胞表面蛋白。我们发现荧光团标记的胶原蛋白模拟肽（CMP）标记GAS细胞，但没有缺少此类蛋白质的大肠杆菌或枯草芽孢杆菌细胞。CMP可能与内源性胶原蛋白形成异源三聚体螺旋，因为CMP的非天然d对映体不会标记GAS细胞。为了鉴定分子靶标，我们使用反向遗传学将编码两个具有胶原样结构域Scl1和Scl2的蛋白质的GAS基因“敲入”枯草芽孢杆菌。荧光CMP标记了这些枯草芽孢杆菌的细胞株。而且，这些菌株与哺乳动物胶原蛋白表面紧密结合。这些数据与链球菌胶原蛋白在伤口床中形成三倍螺旋且胶原蛋白受损的结果一致，因此对微生物毒力具有影响。

更新日期：2020-07-10

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