In this study, recombinase polymerase amplification (RPA) combined with SYBR Green I was developed for the detection of Plasmodium knowlesi. Positive samples were indicated with a green color while negative samples were orange. To increase the efficiency of amplification, an interval mixing step of samples after 3 to 6 min incubation was recommended. Different sets of reaction volumes from 6.25 to 50 µL were tested and the results indicated no differences in detection. RPA's combination with SYBR Green I is fast and easy to perform, hence this method is suitable for use in resource-limited settings.

中文翻译：

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使用重组酶聚合酶扩增 (RPA) 结合 SYBR Green I 检测诺氏疟原虫。


在本研究中，重组酶聚合酶扩增 (RPA) 与 SYBR Green I 相结合，用于检测诺氏疟原虫。阳性样本用绿色表示，而阴性样本用橙色表示。为了提高扩增效率，建议在孵育 3 至 6 分钟后对样品进行间隔混合步骤。对 6.25 至 50 µL 的不同反应体积组进行了测试，结果表明检测结果没有差异。 RPA 与 SYBR Green I 的组合快速且易于执行，因此该方法适合在资源有限的环境中使用。