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Detection of IL-6, IL-10, and TNF-α level in human single-blastocyst conditioned medium using ultrasensitive Single Molecule Array platform and its relationship with embryo quality and implantation: a pilot study.
Journal of Assisted Reproduction and Genetics ( IF 3.2 ) Pub Date : 2020-05-15 , DOI: 10.1007/s10815-020-01805-7
Huixian Zhong 1 , Qing Sun 1 , Peilin Chen 1 , Feng Xiong 1 , Guangui Li 1 , Caiyun Wan 1 , Zhihong Yao 1 , Yong Zeng 1
Affiliation  

PURPOSE The purpose was to investigate the association between embryonic development or implantation and the content of interleukin-6 and 10 (IL-6, IL-10) and tumor necrosis factor-α (TNF-α) in single-blastocyst conditioned medium (SBCM). METHODS Thirty-eight SBCM samples (SBCMs) were collected from blastocysts with different morphological scores. IL-6, IL-10, and TNF-α concentration in 38 SBCMs was detected by Single Molecule Array and compared according to the blastocyst quality: top-quality (TQ) and non-top quality (NTQ), or blastulation time: day 5 (D5) and day 6 (D6). In another experiment, 61 SBCMs were collected from TQ blastocyst transplanted on D5, and IL-6 concentration in SBCM was compared based on whether embryos are implanted or not (implanted and non-implanted). RESULTS In the first experiment, IL-6, IL-10, and TNF-α concentration was not significantly different between the TQ-SBCM and NTQ-SBCM. The D6-SBCM had a higher IL-6 concentration compared with the D5-SBCM, while IL-10 and TNF-α concentration was not significantly different between the D5-SBCM and D6-SBCM. The IL-6 concentration in D5-NTQ or D6-TQ SBCM was higher than that in D5-TQ or D6-NTQ SBCM (P < 0.05), respectively. Furthermore, the spearman analysis demonstrated that IL-6 concentration in SBCM was negatively correlated with the blastocyst quality on D5 and positively correlated with the blastocyst quality on D6. In the second experiment, no significant difference in IL-6 concentration was found between SBCM from implanted and non-implanted blastocyst. CONCLUSION IL-6 concentration in SBCM was associated with embryo quality depending on the blastulation time, although it might not be associated with the blastocyst implantation.

中文翻译:

使用超灵敏单分子阵列平台检测人单胚泡条件培养基中IL-6,IL-10和TNF-α的水平及其与胚胎质量和植入的关系:一项试点研究。

目的旨在研究单胚泡条件培养基(SBCM)中胚胎发育或植入与白细胞介素6和10(IL-6,IL-10)和肿瘤坏死因子-α(TNF-α)含量之间的关系。 )。方法收集38个不同形态学评分的囊胚标本。通过单分子阵列检测38个SBCM中的IL-6,IL-10和TNF-α浓度,并根据胚泡质量:最高质量(TQ)和非最高质量(NTQ)或成囊时间:天进行比较第5天(D5)和第6天(D6)。在另一个实验中,从D5移植的TQ囊胚中收集了61个SBCM,并根据是否植入胚胎(植入和未植入)比较SBCM中IL-6的浓度。结果在第一个实验中,IL-6,IL-10,TQ-SBCM和NTQ-SBCM之间的TNF-α浓度无明显差异。与D5-SBCM相比,D6-SBCM的IL-6浓度更高,而D5-SBCM和D6-SBCM的IL-10和TNF-α浓度无显着差异。D5-NTQ或D6-TQ SBCM中的IL-6浓度分别高于D5-TQ或D6-NTQ SBCM中的IL-6浓度(P <0.05)。此外,spearman分析表明,SBCM中的IL-6浓度与D5上的囊胚质量呈负相关,而与D6上的囊胚质量呈正相关。在第二个实验中,植入的和未植入的胚泡的SBCM之间的IL-6浓度均无显着差异。结论SBCM中IL-6的浓度与胚质量有关,取决于胚泡形成的时间。
更新日期:2020-05-15
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