PURPOSE The purpose was to investigate the association between embryonic development or implantation and the content of interleukin-6 and 10 (IL-6, IL-10) and tumor necrosis factor-α (TNF-α) in single-blastocyst conditioned medium (SBCM). METHODS Thirty-eight SBCM samples (SBCMs) were collected from blastocysts with different morphological scores. IL-6, IL-10, and TNF-α concentration in 38 SBCMs was detected by Single Molecule Array and compared according to the blastocyst quality: top-quality (TQ) and non-top quality (NTQ), or blastulation time: day 5 (D5) and day 6 (D6). In another experiment, 61 SBCMs were collected from TQ blastocyst transplanted on D5, and IL-6 concentration in SBCM was compared based on whether embryos are implanted or not (implanted and non-implanted). RESULTS In the first experiment, IL-6, IL-10, and TNF-α concentration was not significantly different between the TQ-SBCM and NTQ-SBCM. The D6-SBCM had a higher IL-6 concentration compared with the D5-SBCM, while IL-10 and TNF-α concentration was not significantly different between the D5-SBCM and D6-SBCM. The IL-6 concentration in D5-NTQ or D6-TQ SBCM was higher than that in D5-TQ or D6-NTQ SBCM (P < 0.05), respectively. Furthermore, the spearman analysis demonstrated that IL-6 concentration in SBCM was negatively correlated with the blastocyst quality on D5 and positively correlated with the blastocyst quality on D6. In the second experiment, no significant difference in IL-6 concentration was found between SBCM from implanted and non-implanted blastocyst. CONCLUSION IL-6 concentration in SBCM was associated with embryo quality depending on the blastulation time, although it might not be associated with the blastocyst implantation.

中文翻译：

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使用超灵敏单分子阵列平台检测人单胚泡条件培养基中IL-6，IL-10和TNF-α的水平及其与胚胎质量和植入的关系：一项试点研究。

目的旨在研究单胚泡条件培养基（SBCM）中胚胎发育或植入与白细胞介素6和10（IL-6，IL-10）和肿瘤坏死因子-α（TNF-α）含量之间的关系。 ）。方法收集38个不同形态学评分的囊胚标本。通过单分子阵列检测38个SBCM中的IL-6，IL-10和TNF-α浓度，并根据胚泡质量：最高质量（TQ）和非最高质量（NTQ）或成囊时间：天进行比较第5天（D5）和第6天（D6）。在另一个实验中，从D5移植的TQ囊胚中收集了61个SBCM，并根据是否植入胚胎（植入和未植入）比较SBCM中IL-6的浓度。结果在第一个实验中，IL-6，IL-10，TQ-SBCM和NTQ-SBCM之间的TNF-α浓度无明显差异。与D5-SBCM相比，D6-SBCM的IL-6浓度更高，而D5-SBCM和D6-SBCM的IL-10和TNF-α浓度无显着差异。D5-NTQ或D6-TQ SBCM中的IL-6浓度分别高于D5-TQ或D6-NTQ SBCM中的IL-6浓度（P <0.05）。此外，spearman分析表明，SBCM中的IL-6浓度与D5上的囊胚质量呈负相关，而与D6上的囊胚质量呈正相关。在第二个实验中，植入的和未植入的胚泡的SBCM之间的IL-6浓度均无显着差异。结论SBCM中IL-6的浓度与胚质量有关，取决于胚泡形成的时间。