As a potential tool for the biodegradation of nitrogen contaminants, including nitrate, nitrite, and ammonium, in pickled foods with high salinity, the halophilic and denitrifying archaeal strain Halomicrobium sp. ZPS1 was isolated from edible salt particles. Under anaerobic and static culture conditions, Halomicrobium sp. ZPS1 could simultaneously degrade nitrate, nitrite, and ammonium in liquid medium with 18% salinity and generate N2O. To gain insight into these physiological characteristics, the complete genome of Halomicrobium sp. ZPS1 was sequenced to reveal the mechanism of nitrogen metabolism associated with salt-tolerance. The complete genome sequencing revealed a genome size of 3,094,203 bp with a circular chromosome and a GC content of 65.64%. Based on gene annotation, 3191 CDSs, 6 rRNA genes, and 76 tRNA genes were identified. Moreover, 28 genes were annotated as related to salt tolerance, ammonium assimilation, and a truncated denitrification pathway. The annotated functional genes indicate that Halomicrobium sp. ZPS1 could be a candidate strain for the simultaneous removal of nitrate, nitrite, and ammonia in extremely high salt environments.

中文翻译：

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古细菌分离物Halomicrobium sp。的完整基因组序列。ZPS1揭示了高盐条件下的氮代谢特征

具有高盐度和反硝化作用的古细菌菌株Halomicrobium sp作为一种可生物降解含盐量高的腌制食品中氮污染物（包括硝酸盐，亚硝酸盐和铵）的潜在工具。从食用盐颗粒中分离出ZPS1。在厌氧和静态培养条件下，Halomicrobium sp.。ZPS1可以同时降解盐度为18％的液体介质中的硝酸盐，亚硝酸盐和铵盐，并生成N2O。为了深入了解这些生理特征，需要使用Halomicrobium sp。的完整基因组。ZPS1进行了测序，揭示了氮代谢与耐盐性相关的机制。完整的基因组测序结果显示，基因组大小为3,094,203 bp，具有圆形染色体，GC含量为65.64％。基于基因注释，鉴定了3191个CDS，6个rRNA基因和76个tRNA基因。此外，注释了28个基因，它们与耐盐性，铵同化作用和截短的反硝化途径有关。带注释的功能基因表明Halomicrobium sp.。ZPS1可能是在极高盐环境中同时去除硝酸盐，亚硝酸盐和氨的候选菌株。