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A DMP-triggered in vivo maternal haploid induction system in the dicotyledonous Arabidopsis.
Nature Plants ( IF 15.8 ) Pub Date : 2020-05-15 , DOI: 10.1038/s41477-020-0658-7
Yu Zhong 1 , Baojian Chen 1, 2, 3 , Mengran Li 1 , Dong Wang 1 , Yanyan Jiao 1 , Xiaolong Qi 1 , Min Wang 1 , Zongkai Liu 1 , Chen Chen 1 , Yuwen Wang 1 , Ming Chen 1 , Jinlong Li 1 , Zijian Xiao 1 , Dehe Cheng 1 , Wenxin Liu 1 , Kim Boutilier 2 , Chenxu Liu 1 , Shaojiang Chen 1
Affiliation  

Doubled haploid technology using inducer lines carrying mutations in ZmPLA1/MTL/NLD and ZmDMP1-4 has revolutionized traditional maize breeding. ZmPLA1/MTL/NLD is conserved in monocots and has been used to extend the system from maize to other monocots5-7, but no functional orthologue has been identified in dicots, while ZmDMP-like genes exist in both monocots and dicots4,8,9. Here, we report that loss-of-function mutations in the Arabidopsis thaliana ZmDMP-like genes AtDMP8 and AtDMP9 induce maternal haploids, with an average haploid induction rate of 2.1 ± 1.1%. In addition, to facilitate haploid seed identification in dicots, we established an efficient FAST-Red fluorescent marker-based haploid identification system that enables the identification of haploid seeds with >90% accuracy. These results show that mutations in DMP genes also trigger haploid induction in dicots. The conserved expression patterns and amino acid sequences of ZmDMP-like genes in dicots suggest that DMP mutations could be used to develop in vivo haploid induction systems in dicots.

中文翻译:

双子叶拟南芥中的DMP触发的体内孕产妇单倍体诱导系统。

使用携带ZmPLA1 / MTL / NLD和ZmDMP1-4突变的诱导株的双单倍体技术彻底改变了传统的玉米育种。ZmPLA1 / MTL / NLD在单子叶植物中是保守的,已用于将系统从玉米扩展到其他单子叶植物5-7,但在双子叶植物中尚未鉴定出功能直向同源物,而在单子叶植物和双子叶植物中都存在类似ZmDMP的基因4、8、9。 。在这里,我们报道拟南芥ZmDMP样基因AtDMP8和AtDMP9的功能丧失突变诱导母体单倍体,平均单倍体诱导率为2.1±1.1%。此外,为了促进双子叶植物中单倍体种子的鉴定,我们建立了一个基于FAST-Red荧光标记的高效单倍体鉴定系统,该系统能够鉴定> 90%的单倍体种子。这些结果表明,DMP基因中的突变也触发了双子叶植物中的单倍体诱导。双子叶植物中ZmDMP-like基因的保守表达模式和氨基酸序列表明,DMP突变可用于开发双子叶植物体内单倍体诱导系统。
更新日期:2020-05-15
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