Abstract

A new triterpenoid saponin named Anemonside A (1) was isolated from the rhizome of Anemone amurensis (Korsh.) Kom. Its structure was determined by chemical and spectral analysis, including 1D, 2D NMR data, HRESIMS and hydrolysis reaction. The new saponin contains nine sugar units with two O-linked sugar chains, which is relatively rare in natural products. In addition, the cytotoxic activity of Anemonside A was evaluated by against A549 and HepG2 cell lines with the MTT method and showed no cytotoxic activity with IC50 over 100 μM.

中文翻译：

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来自 Anemone amurensis (Korsh.) Kom 的具有 9 个糖单位的新三萜皂苷

摘要

从 Anemone amurensis (Korsh.) Kom 的根茎中分离出一种名为 Anemonside A (1)的新三萜皂苷。其结构由化学和光谱分析确定，包括一维、二维核磁共振数据、HRESIMS和水解反应。新皂素含有九个糖单元和两条O-连接的糖链，这在天然产物中比较少见。此外，使用 MTT 方法通过针对 A549 和 HepG2 细胞系评估了海葵苷A的细胞毒活性，并且在 IC50 超过 100 μM 时没有显示出细胞毒活性。