The medicinal plant Spathodea campanulata P. Beauv. (Bignoniaceae) has been traditionally applied for the prevention and treatment of diseases of the kidney and urinary system, the skin, the gastrointestinal tract, and inflammation in general. The present work shows for the first time how chemical components from this plant inhibit Helicobacter pylori growth by urease inhibition and modulation of virulence factors. The crude extract and the main fractions of S. campanulata bark were tested on H. pylori isolated strains and the active ones were further fractionated. Fractions and sub-fractions of the plant crude extract were characterized by ultra-high-performance liquid chromatographic tandem high resolution-mass spectrometry detection (UHPLC-HRMS). Several phenolics and triterpenoids were identified. Among the sub-fractions obtained, SB2 showed the capacity to inhibit H. pylori urease in a heterologous bacterial model. One additional sub-fraction (SE3) was able to simultaneously modulate the expression of two adhesins (HopZ and BabA) and one cytotoxin (CagA). The flavonol kaempferol was identified as the most interesting compound that deserves further investigation as a new hit for its capacity to modulate H. pylori virulence factors.

中文翻译：

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药用植物Spathodea campanulata P. Beauv对生物活性化合物的新见解。及其对幽门螺杆菌的活性。

药用植物Spathodea campanulata P. Beauv。（Bignoniaceae）传统上已用于预防和治疗肾脏和泌尿系统，皮肤，胃肠道和一般炎症的疾病。本工作首次显示了该植物的化学成分如何通过脲酶抑制和毒力因子调节来抑制幽门螺杆菌的生长。在幽门螺杆菌分离的菌株上测试了桔梗粗皮提取物和主要级分，并将活性菌株进一步分离。通过超高效液相色谱串联高分辨率质谱（UHPLC-HRMS）检测对植物粗提物的馏分和亚馏分进行了表征。确定了几种酚类和三萜类化合物。在获得的子分数中，SB2在异源细菌模型中显示出抑制幽门螺杆菌脲酶的能力。一个额外的子部分（SE3）能够同时调节两种粘附素（HopZ和BabA）和一种细胞毒素（CagA）的表达。黄酮山ka酚被认为是最有趣的化合物，因其调节幽门螺杆菌毒力因子的能力而受到了新的关注。