Changes in environmental temperature influence many aspects of plant metabolism; however, the underlying regulatory mechanisms remain poorly understood. In addition to their role in light perception, phytochromes (PHYs) have been recently recognized as temperature sensors affecting plant growth. In particular, in Arabidopsis (Arabidopsis thaliana), high temperature reversibly inactivates PHYB, reducing photomorphogenesis-dependent responses. Here, we show the role of phytochrome-dependent temperature perception in modulating the accumulation of isoprenoid-derived compounds in tomato (Solanum lycopersicum) leaves and fruits. The growth of tomato plants under contrasting temperature regimes revealed that high temperatures resulted in coordinated up-regulation of chlorophyll catabolic genes, impairment of chloroplast biogenesis, and reduction of carotenoid synthesis in leaves in a PHYB1B2-dependent manner. Furthermore, by assessing a triple phyAB1B2 mutant and fruit-specific PHYA- or PHYB2-silenced plants, we demonstrated that biosynthesis of the major tomato fruit carotenoid, lycopene, is sensitive to fruit-localized PHY-dependent temperature perception. The collected data provide compelling evidence concerning the impact of PHY-mediated temperature perception on plastid metabolism in both leaves and fruit, specifically on the accumulation of isoprenoid-derived compounds.

环境温度的变化会影响植物新陈代谢的许多方面。然而，基本的监管机制仍然知之甚少。除了在光感知中的作用外，植物色素（PHYs）最近还被认为是影响植物生长的温度传感器。特别地，在拟南芥（Arabidopsis thaliana）中，高温可逆地使PHYB失活，从而降低了依赖光形态发生的响应。在这里，我们展示了依赖植物色素的温度感知在调节番茄类异戊二烯衍生化合物（茄子）中的积累中的作用。）叶子和果实。在不同温度下番茄植株的生长表明，高温导致叶绿素分解代谢基因的协同上调，叶绿体生物发生的损害以及以PHYB1B2依赖性方式减少叶片中类胡萝卜素的合成。此外，通过评估三重phyAB1B2突变体和果实特异性PHYA-或PHYB2沉默的植物，我们证明了主要番茄果实类胡萝卜素番茄红素的生物合成对果实局部的PHY依赖温度感知敏感。收集到的数据提供了令人信服的证据，说明PHY介导的温度感知对叶片和果实中质体代谢的影响，特别是对类异戊二烯衍生化合物的积累的影响。