Salt‐sensitive hypertension (SSH) is an independent risk factor for cardiovascular disease. The regulation of long non‐coding RNAs, mRNAs and competing endogenous RNAs (ceRNAs) in the pathogenesis of SSH is uncertain. An RNA microarray was performed to discover SSH‐associated differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs), and 296 DElncRNAs and 44 DEmRNAs were identified, and 247 DElncRNAs and 44 DEmRNAs among these RNAs were included in the coexpression network. The coregulatory network included 23 ceRNA loops, and six hub RNAs (lnc‐ILK‐8:1, lnc‐OTX1‐7:1, lnc‐RCAN1‐6:1, GIMAP8 , SUV420H1 and PIGV ) were identified for further population validation. The ceRNA correlations among lnc‐OTX1‐7:1, hsa‐miR‐361‐5p and GIMAP8 were confirmed in SSH and SRH patients. A larger‐sample validation confirmed that GIMAP8, SUV420H1 and PIGV were differentially expressed between the SSH and SRH groups. In addition, SUV420H1 was included in the SSH screening model, and the area under the curve of the model was 0.720 (95% CI: 0.624‐0.816). Our study explored the transcriptome profiles of SSH and constructed a ceRNA network to help elucidate the mechanism of SSH. In addition, SUV420H1 was identified as a hub element that participates in SSH transcriptional regulation and as a potential biomarker for the early diagnosis of SSH.

中文翻译：

---

ceRNA调控网络的构建和盐敏感性高血压的中枢生物标志物的筛选。

盐敏感性高血压（SSH）是心血管疾病的独立危险因素。长期的非编码RNA，mRNA和竞争性内源性RNA（ceRNA）在SSH的发病机制中的调控尚不确定。使用RNA微阵列发现SSH相关的差异表达的lncRNA（DElncRNA）和mRNA（DEmRNA），鉴定出296个DElncRNA和44个DEmRNA，并将这些RNA中的247个DElncRNA和44个DEmRNA包括在共表达网络中。核心调控网络包括23个ceRNA环，并鉴定了六个集线RNA（lnc-ILK-8：1，lnc-OTX1-7：1，lnc -RCAN1-6：1，GIMAP8，SUV420H1和PIGV）以进行进一步的种群验证。lnc‐OTX1‐7：1，hsa‐miR‐361‐5p和GIMAP8之间的ceRNA相关性已在SSH和SRH患者中得到证实。较大样本验证确认GIMAP8，SUV420H1和PIGV在SSH和SRH组之间差异表达。此外，SSH420筛选模型中包含SUV420H1，该模型的曲线下面积为0.720（95％CI：0.624-0.816）。我们的研究探索了SSH的转录组概况，并构建了一个ceRNA网络来帮助阐明SSH的机制。此外，SUV420H1被认为是参与SSH转录调控的枢纽元件，并且是SSH早期诊断的潜在生物标记。