A Biocatalytic Cascade Reaction to Access a Valuable Long‐Chain ω‐Hydroxy Fatty Acid,ChemCatChem

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A Biocatalytic Cascade Reaction to Access a Valuable Long‐Chain ω‐Hydroxy Fatty Acid
ChemCatChem ( IF 3.8 ) Pub Date : 2020-05-15 , DOI: 10.1002/cctc.202000745
Aileen Becker ₁ , Dominique Böttcher ₁ , Werner Katzer ₂ , Karsten Siems ₂ , Lutz Müller‐Kuhrt ₂ , Uwe T. Bornscheuer ₁

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Environmentally‐friendly processes for the manufacturing of valuable industrial compounds like ω‐hydroxy fatty acids (ω‐OHFAs) are highly desirable. Herein, we present such an approach by establishing a two‐step enzymatic cascade reaction for the production of 2,15,16‐trihydroxy hexadecanoic acid (THA). Starting with the easily accessible natural compound ustilagic acid (UA) that is secreted by the corn smut fungus Ustilago maydis, the recombinantly expressed esterase BS2 from Bacillus subtilis and the commercial β‐glucosidase from almonds were applied yielding 86 % product. Both hydrolases do not require expensive cofactors, making the process economically attractive. Additionally, no harmful solvents are required, so that the product THA can be labelled natural to be used in food and cosmetic products.

中文翻译：

生物催化级联反应以获取有价值的长链ω-羟基脂肪酸

非常需要环保的方法来生产有价值的工业化合物，例如ω-羟基脂肪酸（ω-OHFA）。在本文中，我们通过建立用于生产2,15,16-三羟基十六烷酸（THA）的两步酶促级联反应来介绍这种方法。从玉米黑穗病菌Ustilago maydis分泌的容易获得的天然化合物ustilagic酸（UA）开始，重组表达的枯草芽孢杆菌酯酶BS2和商品β应用杏仁的葡糖苷酶，收率86％。两种水解酶均不需要昂贵的辅因子，因此该方法在经济上具有吸引力。另外，不需要有害溶剂，因此可以将THA产品标记为天然食品，以用于食品和化妆品。

更新日期：2020-05-15

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