A fundamental question is how memory is stored for several weeks and even longer. A long-lasting increase in gene transcription has been suggested to mediate such long-term memory storage. Here, we used contextual fear conditioning in mice to search for lasting transcription that may contribute to long-term memory storage. Our study focussed on hippocampal area CA1, which has been suggested to have a role for at least one week in contextual fear memory. Using an unbiased microarray analysis followed by confirmatory quantitative real-time PCR, we identified an upregulation of two transcription factors, Fosl2 and Nfil3, which lasted for seven days after conditioning. To our knowledge these are the longest transcriptional changes ever detected in the hippocampus after contextual fear conditioning. Thus, our findings suggest novel transcriptional candidates for long-term memory storage.

中文翻译：

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情境恐惧调节后，海马区CA1中的持久转录。

一个基本的问题是如何将内存存储几周甚至更长的时间。已经提出基因转录的持久增加可以介导这种长期的记忆存储。在这里，我们使用小鼠的情境恐惧条件来搜索可能有助于长期记忆存储的持久转录。我们的研究集中在海马区CA1，已被证明在情境恐惧记忆中起着至少一周的作用。使用无偏微阵列分析，然后进行定量实时定量PCR，我们确定了上调后持续7天的两个转录因子Fosl2和Nfil3的上调。据我们所知，这些是经过情境恐惧调节后在海马中发现的最长的转录变化。从而，