Streptococcus agalactiae is considered as a leading case of bacterial infection among neonates. Although relative protection strategies have been performed in many high-income countries, resulting in a massive reduction in the occurrences of early-onset GBS disease, the late-onset disease has not affected. Here, the whole genome of S. agalactiae Guangzhou-SAG036 was sequenced by the Pacific Biosciences Sequel using the P4-C2 chemistry and the continuous long reads were used for de novo assembly using HGAP. Besides, genes prediction and multiply annotation were performed by comparing it with diverse databases. The whole genome has a length of 2,206,504 bp and contains 2162 predicted genes with an average G + C content of 35.85%. Based on the whole genome sequence, 2 large prophages, 20 virulence factors genes, and 8 antibiotic resistant genes were identified. MLST analysis revealed S. agalactiae Guangzhou-SAG036 was identified as ST-17. The virulence factors genes were identified with different functions including adherence, antiphagocytosis, spreading factor, immune evasion, invasion, toxin. Besides, the antibiotic-resistant genes may provide S. agalactiae with resistance to multi-drugs including erythromycin, streptomycin, azithromycin, spiramycin, ampicillin, kanamycin, cationic peptides, and tetracycline. Therefore, the infection of S. agalactiae Guangzhou-SAG036 ST-17 strain maybe caused by the complex virulence factors and multi-drugs resistance. These results contribute to further understand GBS epidemiology and surveillance targets.

中文翻译：

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致病性无乳链球菌广州SAG036的抗药性和毒理学研究。

无乳链球菌被认为是新生儿细菌感染的主要病例。尽管在许多高收入国家已经采取了相对保护策略，从而大大减少了早发性GBS疾病的发生，但迟发性疾病并未受到影响。在这里，利用P4-C2化学方法由Pacific Biosciences Sequel对无乳链球菌广州-SAG036的整个基因组进行了测序，并将连续的长读段用于使用HGAP的从头组装。此外，通过与各种数据库进行比较，进行了基因预测和多重注释。整个基因组的长度为2206504 bp，包含2162个预测基因，平均G + C含量为35.85％。基于整个基因组序列，2个大型噬菌体，20个毒力因子基因，鉴定了8个抗生素抗性基因。MLST分析显示无乳链球菌广州-SAG036被鉴定为ST-17。毒力因子基因具有不同的功能，包括粘附，抗吞噬作用，扩散因子，免疫逃逸，侵袭，毒素。此外，抗生素抗性基因可以使无乳链球菌对包括红霉素，链霉素，阿奇霉素，螺旋霉素，氨苄青霉素，卡那霉素，阳离子肽和四环素在内的多种药物具有抗性。因此，无乳链球菌广州-SAG036 ST-17菌株的感染可能是由复杂的毒力因子和多药耐药性引起的。这些结果有助于进一步了解GBS流行病学和监测目标。毒力因子基因具有不同的功能，包括粘附，抗吞噬作用，扩散因子，免疫逃逸，侵袭，毒素。此外，抗生素抗性基因可以使无乳链球菌对包括红霉素，链霉素，阿奇霉素，螺旋霉素，氨苄青霉素，卡那霉素，阳离子肽和四环素在内的多种药物具有抗性。因此，无乳链球菌广州-SAG036 ST-17菌株的感染可能是由复杂的毒力因子和多药耐药性引起的。这些结果有助于进一步了解GBS流行病学和监测目标。毒力因子基因具有不同的功能，包括粘附，抗吞噬作用，扩散因子，免疫逃逸，侵袭，毒素。此外，抗生素抗性基因可以使无乳链球菌对包括红霉素，链霉素，阿奇霉素，螺旋霉素，氨苄青霉素，卡那霉素，阳离子肽和四环素在内的多种药物具有抗性。因此，无乳链球菌广州-SAG036 ST-17菌株的感染可能是由复杂的毒力因子和多药耐药性引起的。这些结果有助于进一步了解GBS流行病学和监测目标。对多种药物（包括红霉素，链霉素，阿奇霉素，螺旋霉素，氨苄青霉素，卡那霉素，阳离子肽和四环素）具有抗性的无乳杆菌。因此，无乳链球菌广州-SAG036 ST-17菌株的感染可能是由复杂的毒力因子和多药耐药性引起的。这些结果有助于进一步了解GBS流行病学和监测目标。对多种药物（包括红霉素，链霉素，阿奇霉素，螺旋霉素，氨苄青霉素，卡那霉素，阳离子肽和四环素）具有抗性的无乳杆菌。因此，无乳链球菌广州-SAG036 ST-17菌株的感染可能是由复杂的毒力因子和多药耐药性引起的。这些结果有助于进一步了解GBS流行病学和监测目标。