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Efficient entry of budded virions of Autographa californica multiple nucleopolyhedrovirus into Spodoptera frugiperda cells is dependent on dynamin, Rab5, and Rab11.
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2020-05-15 , DOI: 10.1016/j.ibmb.2020.103409 Qi Yue 1 , Jingfeng Li 1 , Ya Guo 1 , Fanye Yan 1 , Ximeng Liu 1 , Gary W Blissard 2 , Zhaofei Li 1
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2020-05-15 , DOI: 10.1016/j.ibmb.2020.103409 Qi Yue 1 , Jingfeng Li 1 , Ya Guo 1 , Fanye Yan 1 , Ximeng Liu 1 , Gary W Blissard 2 , Zhaofei Li 1
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Autographa californica multiple nucleopolyhedrovirus (AcMNPV), a member of the Alphabaculovirus genus of the family Baculoviridae, is an enveloped double-stranded DNA virus. Budded virions (BVs) of AcMNPV enter host cells via clathrin-mediated endocytosis. However, the route of functional intracellular trafficking of AcMNPV BVs during entry is not well established. In the current study, we found that entering BVs were colocalized mainly with cellular Rab5 and Rab11. Expression of dominant-negative (DN) Rab5 and Rab11 or RNAi-mediated down regulation of these two cellular transcripts significantly reduced BVs entry into but not egress from Spodoptera frugiperda cells (Sf9), whereas similar treatments for Rab4 and Rab7 had no apparent effect on virus infection. Combined with data from RNAi knockdowns of dynamin, and dynasore inhibition assays, our results support a model in which AcMNPV BVs enter permissive host cells by clathrin-mediated endocytosis, followed by de-envelopment of BVs predominantly within early and maturing endosomes rather than within late endosomes. Additionally, Rab11 suppression studies suggest the Rab11-dependent recycling endosomal pathway is involved in virion entry.
中文翻译：
产自加州苜蓿的多重核多角体病毒的萌芽病毒粒子能否有效地进入贪食斜纹夜蛾细胞，取决于动力蛋白，Rab5和Rab11。
杆状病毒多核多角体病毒（AcMNPV）是杆状病毒科的杆状病毒属的成员，是一种有包膜的双链DNA病毒。AcMNPV的芽状病毒粒子（BV）通过网格蛋白介导的内吞作用进入宿主细胞。然而，进入过程中AcMNPV BV的功能性细胞内运输途径尚不完善。在当前的研究中，我们发现进入的BV主要与细胞Rab5和Rab11共定位。显性阴性（DN）Rab5和Rab11的表达或RNAi介导的这两个细胞转录产物的下调显着减少了BV进入但不从斜纹夜蛾细胞（Sf9）流出，而类似的Rab4和Rab7处理对病毒感染。结合来自dynamin的RNAi击倒和dynasore抑制试验的数据，我们的结果支持了一个模型，其中AcMNPV BV通过网格蛋白介导的内吞作用进入允许的宿主细胞，然后将BV包埋主要在早期和成熟的内体中，而不是在晚期内体中。此外，Rab11抑制研究表明，Rab11依赖的循环内体途径与病毒体进入有关。
更新日期：2020-05-15
中文翻译：
产自加州苜蓿的多重核多角体病毒的萌芽病毒粒子能否有效地进入贪食斜纹夜蛾细胞，取决于动力蛋白，Rab5和Rab11。
杆状病毒多核多角体病毒（AcMNPV）是杆状病毒科的杆状病毒属的成员，是一种有包膜的双链DNA病毒。AcMNPV的芽状病毒粒子（BV）通过网格蛋白介导的内吞作用进入宿主细胞。然而，进入过程中AcMNPV BV的功能性细胞内运输途径尚不完善。在当前的研究中，我们发现进入的BV主要与细胞Rab5和Rab11共定位。显性阴性（DN）Rab5和Rab11的表达或RNAi介导的这两个细胞转录产物的下调显着减少了BV进入但不从斜纹夜蛾细胞（Sf9）流出，而类似的Rab4和Rab7处理对病毒感染。结合来自dynamin的RNAi击倒和dynasore抑制试验的数据，我们的结果支持了一个模型，其中AcMNPV BV通过网格蛋白介导的内吞作用进入允许的宿主细胞，然后将BV包埋主要在早期和成熟的内体中，而不是在晚期内体中。此外，Rab11抑制研究表明，Rab11依赖的循环内体途径与病毒体进入有关。
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