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Eyes absent in the cockroach panoistic ovaries regulates proliferation and differentiation through ecdysone signalling.
Insect Biochemistry and Molecular Biology ( IF 3.2 ) Pub Date : 2020-05-15 , DOI: 10.1016/j.ibmb.2020.103407
S Ramos 1 , F Chelemen 1 , V Pagone 1 , N Elshaer 2 , P Irles 3 , M D Piulachs 1
Affiliation  

Eyes absent (Eya), is a protein structurally conserved from hydrozoans to humans, for which two basic roles have been reported: it can act as a transcription cofactor and as a protein tyrosine phosphatase. Eya was discovered in the fly Drosophila melanogaster in relation to its function in eye development, and the same function was later reported in other insects. Eya is also involved in insect oogenesis, although studies in this sense are limited to D. melanogaster, which has meroistic ovaries, and where eya mutations abolish gonad formation. In the present work we studied the function of eya in the panoistic ovary of the cockroach Blattella germanica. We show that eya is essential for correct development of panoistic ovaries. In B. germanica, eya acts at different level and in a distinct way in the germarium and the vitellarium. In the germarium, eya contributes to maintain the correct number of somatic and germinal cells by regulating the expression of steroidogenic genes in the ovary. In the vitellarium, eya facilitates follicle cells proliferation and contributes to regulate the cell program, in the context of basal ovarian follicle maturation. Thus, eya-depleted females of B. germanica arrest the growth and maturation of basal ovarian follicles and become sterile.

中文翻译:

蟑螂全景卵巢中不存在的眼睛通过蜕皮激素信号传导来调节增殖和分化。

缺失的眼睛(Eya)是一种结构上从水生动物到人类都保守的蛋白质,据报道有两个基本作用:它可以作为转录辅因子和酪氨酸磷酸酶。在果蝇果蝇中发现了Eya,它与眼睛发育有关,后来在其他昆虫中也报道了同样的功能。尽管在这种意义上的研究仅限于黑腹果蝇(D. melanogaster),它具有卵巢上的节律性卵巢,并且那里的eya突变消除了性腺的形成,但Eya也参与了昆虫的卵子发生。在本工作中,我们研究了eya在德国小Bl Blatella的全景卵巢中的功能。我们表明,大肠卵巢癌对于正确发育大卵巢至关重要。在德国双歧杆菌中,e在细菌和卵菌馆中以不同的水平和不同的方式起作用。在细菌库中 eya通过调节卵巢中类固醇生成基因的表达,有助于维持体细胞和生殖细胞的正确数量。在卵巢基底卵泡成熟的背景下,e卵可促进卵泡细胞增殖并有助于调节细胞程序。因此,贫乏德国芽孢杆菌的雌性动物阻止了基底卵巢卵泡的生长和成熟,并变得不育。
更新日期:2020-05-15
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