Peptides from natural plant proteins have been observed to show various bioactivities in many studies. In this paper, oxidation resistance and anti-inflammatory effect of three millet prolamins peptides (MPP) was evaluated, including peptides with molecular weight below 1kDa obtained from hydrolysate by alcalase and two other peptides: Pro-Phe-Leu-Phe (PFLF) and Ile-Ala-Leu-Leu-Ile-Pro-Phe (IALLIPF), in human keratinocyte HaCaT cells and RAW264.7 murine macrophages. As a result, MPP effectively reduced the production of reactive oxygen species (ROS) as well as malondialdehyde (MDA) but increased the level of glutathione (GSH) in H₂O₂-induced HaCaT cells, showing anti-oxidant bioactivity in cells. To be specific, IALLIPF could more effectively both decrease the ROS and increase GSH than the other two peptides. And PFLF had better activity to reduce MDA production. In addition, MPP were able to significantly alleviate inflammation by inhibiting the yield of nitric oxide (NO) and pro-inflammatory cytokines (TNF-α, interleukin IL-6 and IL-1β) in (lipopolysaccharide) LPS-stimulated RAW264.7 murine macrophages. Moreover, these findings suggested that MPP pre-treatment lowered the level of p-IκB and p65 by suppressing the translocation of NF-kB to cell nucleus. Besides, this effect might be related to the inhibition of MAPK pathway as well. Therefore, this study suggested that MPP could be a possible anti-inflammatory alternative for the therapy of inflammatory related illness.

在许多研究中，已经观察到来自天然植物蛋白的肽显示出各种生物活性。本文评估了三种小米谷醇溶蛋白肽（MPP）的抗氧化性和抗炎作用，包括通过碱解酶从水解产物中获得的分子量低于1kDa的肽和其他两种肽：Pro-Phe-Leu-Phe（PFLF）和人角质形成细胞HaCaT细胞和RAW264.7鼠巨噬细胞中的Ile-Ala-Leu-Leu-Ile-Pro-Phe（IALLIPF）。结果，MPP有效减少了活性氧（ROS）和丙二醛（MDA）的产生，但增加了H ₂ O ₂中谷胱甘肽（GSH）的水平。诱导的HaCaT细胞，在细胞中显示出抗氧化的生物活性。具体而言，IALLIPF可以比其他两种肽更有效地降低ROS和增加GSH。PFLF具有更好的减少MDA产生的活性。此外，MPP能够通过抑制脂多糖（LPS）刺激的RAW264.7小鼠中一氧化氮（NO）和促炎性细胞因子（TNF-α，白介素IL-6和IL-1β）的产生来显着缓解炎症。巨噬细胞。此外，这些发现表明，MPP预处理通过抑制NF-kB向细胞核的转运降低了p-IκB和p65的水平。此外，该作用也可能与抑制MAPK途径有关。因此，这项研究表明，MPP可能是治疗炎症相关疾病的一种抗炎替代品。