Purpose

Lid wiper epitheliopathy (LWE) is a clinical sign that has been associated with dry eye disease. This study used a semi-automated method to identify the effect of drop instillation and post-dye viewing time on the absorption of lissamine green (LG) and sodium fluorescein (NaFl) on the upper eyelid in order to ascertain the optimal identification for LWE assessment.

Methods

In 37 participants with LWE, 1-drop of 1% LG (10 μL) was applied to the superior bulbar conjunctiva in the right eye, and photographs of the lid margin were taken 1, 3, and 5 min after instillation. Measurements were repeated in the same eye following instillations of 2-drops of 1% LG. The same procedures were followed for application of 2% NaFl (2 μL) to the left eye. Staining area was determined using software to detect and measure dye-stained images. Analysis used a linear mixed model with fixed effects of time, number of drops and their interaction.

Results

For LG, multivariate analysis showed that time of drop instillation was significant (p = 0.0091) as was the area of staining in the 2-drop versus 1-drop condition (p < 0.0001). For NaFl, there was a significant effect of time (p < 0.0001), drops (p < 0.0001), and a time/drops interaction (p < 0.0134), suggesting that both time and number of drops are important.

Conclusion

A single drop of dye is insufficient to reveal the full extent of LWE staining. A 2-drop instillation is recommended and observation is recommended between 1 and 5 min (LG) and between 3 and 5 min (NaFl).

中文翻译：

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盖子擦拭器上皮病鉴定的最佳方法

目的

盖子擦拭器上皮病 (LWE) 是一种与干眼病相关的临床症状。本研究使用半自动方法来确定滴注和染色后观察时间对上眼睑丽丝胺绿 (LG) 和荧光素钠 (NaFl) 吸收的影响，以确定 LWE 评估的最佳识别方式.

方法

在 37 名 LWE 参与者中，将 1 滴 1% LG (10 μL) 滴入右眼的上球结膜，并在滴注后 1、3 和 5 分钟拍摄眼睑边缘的照片。在滴注 2 滴 1% LG 后，在同一只眼睛中重复测量。遵循相同的程序将 2% NaFl (2 μL) 应用到左眼。使用软件检测和测量染料染色图像来确定染色面积。分析使用了一个线性混合模型，它具有固定的时间、滴数及其相互作用的影响。

结果

对于 LG，多变量分析表明滴注时间很重要 ( p = 0.0091)，2 滴与 1 滴条件下的染色面积 ( p < 0.0001) 也是如此。对于 NaFl，时间 ( p < 0.0001)、滴 ( p < 0.0001) 和时间/滴相互作用 ( p < 0.0134)有显着影响，表明时间和滴数都很重要。

结论

一滴染料不足以揭示 LWE 染色的全部范围。建议滴注 2 滴，建议在 1 到 5 分钟 (LG) 和 3 到 5 分钟 (NaFl) 之间进行观察。