Saccharum spontaneum L. is one of the most drought-resistant plants among sugarcane breeding materials. To elucidate the internal molecular mechanisms that occur under drought stress conditions in drought-resistant clone 13-13 and drought-sensitive clone 84-261 of S. spontaneum, functional genes closely associated with drought resistance were identified to improve the study and utilization of stress resistance genes in S. spontaneum. High-throughput transcriptome sequencing analyses were performed on the 13-13 and 84-261 clones of this species as well as on control leaves of these materials after 7 days of drought stress. The genes in these two samples that displayed very significant differential expression mainly participated in metabolic activities associated with abiotic stress or adversity, including plant hormone signal transduction, glycolysis/gluconeogenesis, starch and sucrose metabolism, photosynthesis, and oxidative phosphorylation. This study identified some key DEGs for drought resistance, such as DEGs involved in the osmotic regulator, ROS removal system, toxin-degrading enzymes, secondary metabolism, signaling, transcription factors, and biotic and abiotic stresses. We speculated that these genes may have played an important role to resist drought in S. spontaneum. This study identified changes in gene expression and obtained functional information on DEGs in two drought-stressed samples and control. The results showed that the 13-13 had more DEGs than the 84-261. In addition, 13-13 had more down-regulated genes than up-regulated genes, whereas 84-261 had more up-regulated genes than down-regulated genes. These results confirm that drought-resistant S. spontaneum uses the constitutive expression of certain genes to respond to drought stress, whereas drought-sensitive S. spontaneum expresses drought-resistant genes only during drought stress. Thus, the S. spontaneum clone 13-13 exhibited stronger drought resistance than clone 84-261. The results of this study indicate that the response of S. spontaneum to drought stress involves the coordinated regulation of multiple genes and multiple biological metabolic processes and suggest that changes in gene expression might be the major regulatory method through which this species copes with drought stress.

中文翻译：

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不同基因型自发蔗糖抗旱转录组的比较分析。

Saccharum spontaneum L.是甘蔗育种材料中最耐干旱的植物之一。为阐明自发链球菌抗旱克隆13-13和干旱敏感克隆84-261在干旱胁迫条件下发生的内部分子机制，鉴定了与干旱抗性密切相关的功能基因，以改善胁迫的研究和利用。自发链球菌抗性基因。在干旱胁迫7天后，对该物种的13-13和84-261克隆以及这些材料的对照叶片进行了高通量转录组测序分析。这两个样品中表现出非常显着差异表达的基因主要参与了与非生物胁迫或逆境有关的代谢活动，包括植物激素信号转导，糖酵解/糖异生，淀粉和蔗糖代谢，光合作用和氧化磷酸化。这项研究确定了一些关键的抗旱DEG，例如参与渗透调节剂，ROS去除系统，毒素降解酶，次级代谢，信号传导，转录因子以及生物和非生物胁迫的DEG。我们推测这些基因可能在抵抗干旱中起了重要作用。自发链球菌。这项研究确定了基因表达的变化，并获得了两个干旱胁迫样品和对照中DEG的功能信息。结果显示13-13比84-261具有更多的DEG。另外，13-13具有下调基因比上调基因多，而84-261具有上调基因比下调基因多。这些结果证实，抗旱的自发链霉菌利用某些基因的组成型表达来响应干旱胁迫，而对干旱敏感的自发链霉菌仅在干旱胁迫期间表达抗旱的基因。因此，自发链球菌克隆13-13显示出比克隆84-261更强的抗旱性。这项研究的结果表明，自发性沙门氏菌对干旱胁迫涉及多个基因和多个生物代谢过程的协调调节，并表明基因表达的改变可能是该物种应对干旱胁迫的主要调节方法。